Capín-Gutiérrez N, Talamás-Rohana P, González-Robles A, Lavalle-Montalvo C, Kourí J B
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), México D.F., México.
Histol Histopathol. 2004 Oct;19(4):1125-32. doi: 10.14670/HH-19.1125.
Morphological and functional changes of chondrocytes are typical in OA cartilage. In this work, we have described noteworthy changes in intermediate filaments cytoskeleton evidenced by transmission electron microscopy. Alterations in the distribution as well as in the content of vimentin, actin, and tubulin have been described by specific fluorescence labelling of each cytoskeletal component and confocal analysis. Normal vs OA cartilages showed a reduction in the percentage of labelled chondrocytes of 37.1% for vimentin, 4.7% for actin, and 20.1% for tubulin. Statistical analysis of fluorescence intensities (mean % +/- SEM) between normal and OA rat cartilage revealed a highly significant difference in vimentin, a significant difference in tubulin, and a non-significant difference in actin. Moreover, by western blot, altered electrophoretic patterns were observed mainly for vimentin and tubulin in OA cartilage in comparison with normal cartilage. These results allow us to suggest that substantial changes in vimentin and tubulin cytoskeleton of chondrocytes might be involved in OA pathogenesis.
软骨细胞的形态学和功能变化在骨关节炎（OA）软骨中很典型。在这项研究中，我们描述了通过透射电子显微镜观察到的中间丝细胞骨架的显著变化。通过对每个细胞骨架成分进行特异性荧光标记和共聚焦分析，描述了波形蛋白、肌动蛋白和微管蛋白在分布及含量上的改变。正常软骨与OA软骨相比，波形蛋白标记的软骨细胞百分比降低了37.1%，肌动蛋白降低了4.7%，微管蛋白降低了20.1%。对正常和OA大鼠软骨之间的荧光强度（平均百分比±标准误）进行统计分析发现，波形蛋白有极显著差异，微管蛋白有显著差异，肌动蛋白无显著差异。此外，通过蛋白质印迹法，与正常软骨相比，在OA软骨中主要观察到波形蛋白和微管蛋白的电泳图谱发生了改变。这些结果使我们认为，软骨细胞波形蛋白和微管蛋白细胞骨架的实质性变化可能与OA的发病机制有关。
