凝缩素 I 与脊椎动物染色体的结构和基因调控区域结合。
Condensin I associates with structural and gene regulatory regions in vertebrate chromosomes.
机构信息
1] Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Melbourne, Victoria 3052, Australia [2] Department of Paediatrics, University of Melbourne, Parkville, Melbourne, Victoria 3052, Australia.
出版信息
Nat Commun. 2013;4:2537. doi: 10.1038/ncomms3537.
The condensin complex is essential for correct packaging and segregation of chromosomes during mitosis and meiosis in all eukaryotes. To date, the genome-wide location and the nature of condensin-binding sites have remained elusive in vertebrates. Here we report the genome-wide map of condensin I in chicken DT40 cells. Unexpectedly, we find that condensin I binds predominantly to promoter sequences in mitotic cells. We also find a striking enrichment at both centromeres and telomeres, highlighting the importance of the complex in chromosome segregation. Taken together, the results show that condensin I is largely absent from heterochromatic regions. This map of the condensin I binding sites on the chicken genome reveals that patterns of condensin distribution on chromosomes are conserved from prokaryotes, through yeasts to vertebrates. Thus in three kingdoms of life, condensin is enriched on promoters of actively transcribed genes and at loci important for chromosome segregation.
凝缩复合体对于所有真核生物的有丝分裂和减数分裂中染色体的正确包装和分离是必不可少的。迄今为止，脊椎动物中凝缩复合体结合位点的全基因组定位和性质仍然难以捉摸。在这里，我们报道了鸡 DT40 细胞中凝缩复合体 I 的全基因组图谱。出乎意料的是，我们发现凝缩复合体 I 在有丝分裂细胞中主要结合到启动子序列上。我们还在着丝粒和端粒处发现了明显的富集，突出了该复合体在染色体分离中的重要性。总之，结果表明凝缩复合体 I 主要不存在于异染色质区域。该鸡基因组上凝缩复合体 I 结合位点图谱表明，染色体上凝缩复合体的分布模式从原核生物、酵母到脊椎动物是保守的。因此，在生命的三个王国中，凝缩复合体在活跃转录基因的启动子和对染色体分离重要的基因座上富集。
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