常规微量法测定人血浆中维生素 K1。
Routine micromethod for the determination of vitamin K1 in human plasma.
机构信息
*Department of Clinical Pharmacology, University Medical Centre of the Georg August University Göttingen, Göttingen, Germany; and †Institute of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Ulm, Germany.
出版信息
Ther Drug Monit. 2013 Dec;35(6):859-62. doi: 10.1097/FTD.0b013e31828eff38.
BACKGROUND
A selective and sensitive high-performance liquid chromatographic method with fluorescence detection after postcolumn reduction is described for the routine measurement of vitamin K1 in plasma samples of 100 µL of volume.
METHODS
Liquid-liquid extraction was used for the sample preparation with vitamin K2 as an internal standard. For the chromatographic separation, a standard C18 column was applied. The calibration range used was from 2 to 500 ng/mL.
RESULTS
At the low level (5 ng/mL), a coefficient of variation of 14.9% was observed interday in the quality control samples, whereas at the intermediate (50 ng/mL) and high (200 ng/mL) levels such of 6.8% and 8.7% were found.
CONCLUSIONS
The method has proven robust and convenient for the analysis of samples from clinical pharmacological studies.
背景
本文描述了一种选择性和灵敏的高效液相色谱荧光检测法，可用于常规测量 100µL 体积的血浆样品中的维生素 K1。
方法
采用液-液萃取法进行样品制备，以维生素 K2 作为内标。色谱分离采用标准 C18 柱。所用的校准范围为 2 至 500ng/mL。
结果
在低浓度（5ng/mL）下，质控样品的日内变异系数为 14.9%，而在中浓度（50ng/mL）和高浓度（200ng/mL）下，变异系数分别为 6.8%和 8.7%。
结论
该方法已被证明在临床药理学研究中分析样品时具有强大而方便的特点。
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