各种生物基质中维生素 K 测定的定量方法简述。
A concise review of quantification methods for determination of vitamin K in various biological matrices.
机构信息
Clinical Pharmacological Laboratory, Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, United States.
Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India.
出版信息
J Pharm Biomed Anal. 2019 May 30;169:133-141. doi: 10.1016/j.jpba.2019.03.006. Epub 2019 Mar 5.
Vitamin K is an essential nutrient in the body and involved in numerous physiological and pathophysiological functions. Both the lack and surplus of vitamin K can put human health at risk. Therefore, it becomes necessary to monitor vitamin K concentrations in different biomatrices through establishing sensitive and specific analytical methods. This review collectively describes an updated overview of the sample pretreatment methodologies and methods for quantitative determination of vitamin K that have been used in last two decades. High Performance Liquid Chromatography (HPLC) is commonly utilized as a standard for separation of vitamin K in combination with different detection including spectroscopic, spectrometric, fluorometric and mass spectroscopy. Recent progress in sample pretreatment technologies and quantitation methodologies have enhanced the ability to identify and quantitate vitamin K in biomatrices to further advance our understanding of the role of this vitamin in human health and disease.
维生素 K 是人体内必需的营养物质，参与多种生理和病理生理功能。维生素 K 的缺乏和过剩都会对人类健康造成威胁。因此，通过建立灵敏、特异的分析方法，监测不同生物基质中维生素 K 的浓度变得尤为重要。本综述综合描述了过去二十年中用于定量测定维生素 K 的样品预处理方法和分析方法的最新进展。高效液相色谱法（HPLC）通常与不同的检测方法（包括光谱法、光谱法、荧光法和质谱法）结合使用，作为分离维生素 K 的标准。样品预处理技术和定量方法的最新进展提高了在生物基质中鉴定和定量维生素 K 的能力，从而进一步加深了我们对这种维生素在人类健康和疾病中的作用的理解。
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