Finkelstein R A, Burks M F, Rieke L C, McDonald R J, Browne S K, Dallas W S
Dev Biol Stand. 1985;59:51-62.
Single amino acid substitutions, introduced by genetic engineering, significantly modify the behavior of the B-subunits of the cholera/coli enterotoxin family in SDS-PAGE and also markedly affect the reactivity of the proteins with mouse hybridoma-derived monoclonal antibodies raised against H-LT. The results indicate that single amino acids play an important role in defining epitopes in these proteins.
通过基因工程引入的单个氨基酸取代，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中显著改变了霍乱/大肠杆菌肠毒素家族B亚基的行为，并且也明显影响了这些蛋白质与针对热不稳定肠毒素（H-LT）产生的小鼠杂交瘤来源的单克隆抗体的反应性。结果表明，单个氨基酸在确定这些蛋白质中的表位方面起着重要作用。
