Robb M, Nichols J C, Whoriskey S K, Murphy J R
Infect Immun. 1982 Oct;38(1):267-72. doi: 10.1128/iai.38.1.267-272.1982.
Hybridoma cell lines which produced monoclonal antibodies against cholera toxin were isolated. These cell lines were detected with an enzyme-linked immunosorbent assay screening procedure with purified cholera toxin or subunit A of cholera toxin. Seven cell lines were characterized with respect to their reactivity with cholera toxin subunits by Western blot analysis. Five clones produced antibodies which were directed against subunit A, and two clones produced antibodies which reacted with subunit B. These antibodies were also characterized by Western blot analysis for reactivity with the heat-labile enterotoxin produced by porcine and human enterotoxinogenic strains of Escherichia coli. Monoclonal antibodies which reacted with subunit A of cholera toxin also reacted with subunit A of both porcine and human heat-labile enterotoxins. In contrast, monoclonal antibodies to subunit B of cholera toxin did not react with subunit B of the heat-labile enterotoxin. Antibodies directed against subunit B neutralized the biological activity of cholera toxin in vitro in the S49 mouse lymphosarcoma assay. In contrast to polyclonal anti-subunit A antisera, monoclonal anti-subunit A from four of five clones had small but measurable neutralizing capacities in vitro.
分离出了产生抗霍乱毒素单克隆抗体的杂交瘤细胞系。这些细胞系通过用纯化的霍乱毒素或霍乱毒素A亚基进行的酶联免疫吸附测定筛选程序进行检测。通过蛋白质印迹分析对七个细胞系与霍乱毒素亚基的反应性进行了表征。五个克隆产生了针对A亚基的抗体，两个克隆产生了与B亚基反应的抗体。还通过蛋白质印迹分析对这些抗体与猪和人产肠毒素大肠杆菌菌株产生的不耐热肠毒素的反应性进行了表征。与霍乱毒素A亚基反应的单克隆抗体也与猪和人不耐热肠毒素的A亚基反应。相反，针对霍乱毒素B亚基的单克隆抗体不与不耐热肠毒素的B亚基反应。针对B亚基的抗体在S49小鼠淋巴瘤测定中体外中和了霍乱毒素的生物学活性。与多克隆抗A亚基抗血清相反，五个克隆中有四个的单克隆抗A亚基在体外具有小但可测量的中和能力。
