Zhurova Mariia, Akabutu John, Acker Jason
Department of Laboratory Medicine and Pathology, University of Alberta, 8249-114 Street, Edmonton, AB, Canada T6G 2R8.
J Blood Transfus. 2012;2012:102809. doi: 10.1155/2012/102809. Epub 2011 Dec 14.
Red blood cells (RBCs) from cord blood contain fetal hemoglobin that is predominant in newborns and, therefore, may be more appropriate for neonatal transfusions than currently transfused adult RBCs. Post-collection, cord blood can be stored at room temperature for several days before it is processed for stem cells isolation, with little known about how these conditions affect currently discarded RBCs. The present study examined the effect of the duration cord blood spent at room temperature and other cord blood characteristics on cord RBC quality. RBCs were tested immediately after their isolation from cord blood using a broad panel of quality assays. No significant decrease in cord RBC quality was observed during the first 65 hours of storage at room temperature. The ratio of cord blood to anticoagulant was associated with RBC quality and needs to be optimized in future. This knowledge will assist in future development of cord RBC transfusion product.
脐带血中的红细胞含有胎儿血红蛋白，这在新生儿中占主导地位，因此，与目前输注的成人红细胞相比，可能更适合新生儿输血。采集后，脐带血在处理以分离干细胞之前可在室温下储存数天，对于这些条件如何影响目前被丢弃的红细胞知之甚少。本研究考察了脐带血在室温下放置的时长以及其他脐带血特征对脐带血红细胞质量的影响。从脐带血中分离出红细胞后，立即使用一系列广泛的质量检测方法对其进行检测。在室温储存的前65小时内，未观察到脐带血红细胞质量有显著下降。脐带血与抗凝剂的比例与红细胞质量相关，未来需要进行优化。这些知识将有助于脐带血红细胞输血产品的未来开发。
