Department of Research and Development, Sanquin Blood Bank, Plesmanlaan 1a, Leiden, the Netherlands.
Vox Sang. 2011 May;100(4):367-73. doi: 10.1111/j.1423-0410.2010.01440.x. Epub 2010 Nov 23.
The use of umbilical cord blood (UCB) for transfusion purposes has gained interest the past years. UCB transfusion could serve premature infants, who often need transfusions early in life.
We investigated the suitability of different storage media. UCB was collected after 25 0/7--35 6/7 gestational weeks and centrifuged to concentrate red cells subsequently stored in saline-adenine-glucose-mannitol (SAGM), or in additive solution-3 (AS-3), or stored as whole blood in citrate-phosphate-dextrose-adenine-1. Quality parameters were measured at 7 day intervals during 35 days and compared to the standard RBC product.
White-blood-cell- and platelet counts were higher in the UCB products. In the fractionated units, haemolysis remained below 1·0% in 64% after 14 days, and in 30% after 21 days. Storage in SAGM or AS-3 showed similar quality. Whole blood UCB showed better pH and haemolysis rates after 21 days.
UCB can be processed into autologous products for premature infants. Shelf-life is limited to 14-21 days and compares unfavourably to stored whole blood. Considering the early transfusion needs in these infants, a short shelf-life would not be a practical objection.
在过去的几年中，人们对使用脐带血（UCB）进行输血产生了兴趣。UCB 输血可用于早产儿，早产儿通常在生命早期需要输血。
我们研究了不同储存介质的适用性。UCB 在妊娠 25 0/7-35 6/7 周后收集，并离心浓缩红细胞，随后储存在生理盐水-腺嘌呤-葡萄糖-甘露醇（SAGM）中，或在添加剂溶液-3（AS-3）中，或储存在柠檬酸-磷酸盐-葡萄糖-腺嘌呤-1 中作为全血。在 35 天内每隔 7 天测量一次质量参数，并与标准 RBC 产品进行比较。
UCB 产品中的白细胞和血小板计数较高。在分离的单位中，在 14 天后，64%的红细胞溶血率低于 1.0%，在 21 天后，30%的红细胞溶血率低于 1.0%。SAGM 或 AS-3 储存的质量相似。在 21 天后，全血 UCB 的 pH 和溶血率更好。
UCB 可加工成早产儿的自体产品。保质期限于 14-21 天，与储存的全血相比不占优势。考虑到这些婴儿的早期输血需求，短的保质期不会成为实际的障碍。
