Sakulramrung R, Domingue G J
Immunobiology. 1985 May;169(4):372-88. doi: 10.1016/S0171-2985(85)80018-8.
Little information is available on the antigenic and immunogenic properties of an E. coli rough mutant: J5 derived from E. coli 0111:B4, and the relationship of J5 to other cross-reacting antigens of Enterobacteriaceae. Subcellular fractions of J5 and various antigen preparations were tested against antisera to Enterobacterial Common Antigen (ECA-Kunin) and to the Re mutant of S. minnesota (R595). ECA-Kunin was demonstrated in all subcellular fractions of the J5 mutant by means of indirect hemagglutination and by hemagglutination inhibition tests. This common antigen was separable from J5 LPS by ethanol fractionation and by phenol-chloroform-petroleum ether extraction. Treatment with alkali destroyed the hemagglutinating reactivity of ECA-Kunin and revealed the complex nature of J5 surface antigens; an alkalinized 20p30 fraction (containing cell wall components) contained both specific J5 antigen and an antigen which cross-reacted with S. minnesota and S. typhimurium. This antigen was shown by absorption studies to be a new common antigen other than Re LPS or ECA-Kunin. Studies of J5 LPS by ELISA demonstrated that there was a shared cross-reactive immunodeterminant between the glycolipids of J5 and Re. Accordingly, heat-killed J5 preparations were complex vaccines which were able to elicit an antibody response with at least two specificities: ECA-Kunin and the specific J5 antigen.
关于大肠杆菌粗糙突变体J5（源自大肠杆菌0111:B4）的抗原性和免疫原性特性，以及J5与肠杆菌科其他交叉反应抗原之间的关系，目前所知甚少。用抗肠杆菌共同抗原（ECA - Kunin）血清和明尼苏达沙门氏菌Re突变体（R595）血清检测了J5的亚细胞组分和各种抗原制剂。通过间接血凝试验和血凝抑制试验在J5突变体的所有亚细胞组分中证实了ECA - Kunin。这种共同抗原可通过乙醇分级分离和苯酚 - 氯仿 - 石油醚萃取从J5脂多糖中分离出来。用碱处理破坏了ECA - Kunin的血凝反应性，并揭示了J5表面抗原的复杂性质；碱化的20p30组分（含有细胞壁成分）既含有特异性J5抗原，也含有一种与明尼苏达沙门氏菌和鼠伤寒沙门氏菌交叉反应的抗原。吸收研究表明，这种抗原是一种不同于Re脂多糖或ECA - Kunin的新的共同抗原。通过ELISA对J5脂多糖的研究表明，J5和Re的糖脂之间存在共同的交叉反应免疫决定簇。因此，热灭活的J5制剂是复合疫苗，能够引发至少具有两种特异性的抗体反应：ECA - Kunin和特异性J5抗原。
