Sakulramrung R, Domingue G J
J Infect Dis. 1985 Jun;151(6):995-1004. doi: 10.1093/infdis/151.6.995.
The potential immunoprotective role of antiserum to an Escherichia coli J5 mutant derived from E. coli O111:B4 was demonstrated in an experimental mouse model. Overwhelming bacterial inocula masked the effects of cross-reactive immunoprotection due to antiserum to strain J5. Enhanced bacterial clearance was observed in mice receiving antiserum to strain J5 in sublethal infections but not from lethal doses. Incorporation of hemoglobin with the bacterial inocula decreased the 50% lethal dose of challenge organisms, allowing the demonstration of protective activity of antiserum to strain J5 in lethal infection. Pretreatment of mice with antiserum to strain J5 did not protect against lethal doses of endotoxin. The protective factor was demonstrated by exhaustive adsorption experiments to be an antibody specific for strain J5 lipopolysaccharide. The protective activity of antiserum to strain J5 was abolished only after adsorption with strain J5 lipopolysaccharide but not with Salmonella typhimurium mutants with or without enterobacterial common antigen.
在实验小鼠模型中证实了源自大肠杆菌O111:B4的大肠杆菌J5突变体抗血清的潜在免疫保护作用。大量的细菌接种掩盖了由于J5菌株抗血清引起的交叉反应性免疫保护的作用。在亚致死性感染中，接受J5菌株抗血清的小鼠观察到细菌清除增强，但在致死剂量感染中未观察到。将血红蛋白与细菌接种物混合可降低攻击生物体的50%致死剂量，从而能够证明J5菌株抗血清在致死性感染中的保护活性。用J5菌株抗血清预处理小鼠不能抵御致死剂量的内毒素。通过彻底的吸附实验证明，保护因子是一种针对J5菌株脂多糖的特异性抗体。仅在用J5菌株脂多糖吸附后，J5菌株抗血清的保护活性才被消除，而用有或没有肠杆菌共同抗原的鼠伤寒沙门氏菌突变体吸附则不会消除其保护活性。
