Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, University of Konstanz , Universitätsstrasse 10, 78457 Konstanz, Germany.
J Am Chem Soc. 2013 Oct 23;135(42):15667-9. doi: 10.1021/ja405346s. Epub 2013 Oct 9.
Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.
功能性核苷酸在许多前沿的生物分子技术中都很重要。通常需要连续掺入几个修饰核苷酸。本研究对 KlenTaq DNA 聚合酶（一种水生栖热菌 DNA 聚合酶的截短形式）进行了结构研究，首次揭示了多核苷酸修饰是如何被 DNA 聚合酶处理的，从而有助于理解这些酶与人工底物相互作用时的功能。
