State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Exp Parasitol. 2013 Nov;135(3):606-10. doi: 10.1016/j.exppara.2013.09.019. Epub 2013 Oct 1.
Newly synthesized epitopes are one of the most promising antigens for the development of diagnostic kits and peptide vaccines. Very little is known about the B cell epitopes on GRA1 of Toxoplasma gondii, which are recognized by the humoral immune response in pigs. In this study, epitopes derived from GRA1 of T. gondii were identified using synthetic peptide techniques and bioinformatics. Three (PG10, PG13 and PG18) out of the eighteen peptides tested were recognized by all pig sera from different time points after infection, and the other peptides were recognized by select sera from various time points after infection. Our data indicate that many regions of GRA1, and in particular, the regions represented by the peptides PG10, PG13 and PG18, are involved in the pig antibody response. The identification of specific epitopes targeted by the host antibody response is important both for understanding the natural response to infection and for the development of epitope-based marker vaccines and diagnostic tools for toxoplasmosis.
新合成的表位是开发诊断试剂盒和肽疫苗最有前途的抗原之一。关于刚地弓形虫 GRA1 上的 B 细胞表位，人们知之甚少，这些表位是猪的体液免疫反应所识别的。在这项研究中，使用合成肽技术和生物信息学方法鉴定了刚地弓形虫 GRA1 上的表位。在检测的 18 种肽中，有 3 种（PG10、PG13 和 PG18）被感染后不同时间点的所有猪血清识别，而其他肽则被不同时间点的选择血清识别。我们的数据表明，GRA1 的许多区域，特别是由肽 PG10、PG13 和 PG18 代表的区域，参与了猪的抗体反应。鉴定宿主抗体反应所针对的特定表位，对于了解对感染的自然反应以及开发基于表位的弓形虫病疫苗和诊断工具都很重要。
