氯喹可损害干扰素诱导的抗病毒状态，而不影响2',5'-寡腺苷酸合成酶。
Chloroquine impairs the interferon-induced antiviral state without affecting the 2',5'-oligoadenylate synthetase.
作者信息
Chelbi-Alix M K, Thang M N
出版信息
J Biol Chem. 1985 Jul 5;260(13):7960-4.
Chloroquine, a weak base which raises the pH in acidic cellular compartments such as lysosomes and endosomes, counteracts the induction by interferon of the antiviral state but not that of the 2',5'-oligoadenylate synthetase in three different types of cell lines (MDBK, WISH, and L929). Active interferon is recovered in crude extracts of cells which have been treated with interferon and chloroquine together, but not in extracts of cells treated with interferon alone, indicating that chloroquine has inhibited the intralysosomal proteolysis of interferon. A low pH-dependent event in the intracellular fate of interferon (perhaps its intralysosomal degradation) is, therefore, necessary for the establishment of the antiviral state but not for the induction of the 2',5'-oligoadenylate synthetase.
氯喹是一种弱碱，可提高溶酶体和内体等酸性细胞区室的pH值，它能抵消干扰素诱导的抗病毒状态，但在三种不同类型的细胞系（MDBK、WISH和L929）中却不能抵消2',5'-寡腺苷酸合成酶的诱导作用。在用干扰素和氯喹共同处理的细胞粗提物中可回收活性干扰素，而在用干扰素单独处理的细胞提取物中则无法回收，这表明氯喹抑制了干扰素在溶酶体内的蛋白水解。因此，干扰素细胞内命运中一个低pH依赖性事件（可能是其在溶酶体内的降解）对于建立抗病毒状态是必要的，但对于诱导2',5'-寡腺苷酸合成酶则不是必需的。
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