Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe 85287-6501, USA.
Biomaterials. 2013 Dec;34(38):9779-88. doi: 10.1016/j.biomaterials.2013.09.031. Epub 2013 Oct 1.
A dual glucose and oxygen sensor in a polymer format was developed. The dual sensor composed of a blue emitter as the glucose probe, a red emitter as an oxygen probe, and a yellow emitter as a built-in reference probe which does not respond to either glucose or oxygen. All the three probes were chemically immobilized in a polyacrylamide-based matrix. Therefore, the dual sensor possesses three well separated emission colors and ratiometric approach is applicable for analysis of the glucose and oxygen concentration at biological conditions. The sensor was applied for real-time monitoring of glucose and oxygen consumption of bacterial cells, Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis), and mammalian cells of mouse macrophage J774 and human cervical cancer HeLa cell lines. On the other hand, in order to achieve satisfactory sensing performance for glucose, compositions of the matrices among poly(2-hydroxyethyl methacrylate), polyacrylamide, and poly(6-aminohexyl methacrylamide) which is a linker polymer for grafting the glucose probe, were optimized.
研制了一种聚合物形式的双葡萄糖和氧传感器。该双传感器由蓝色发射器作为葡萄糖探针、红色发射器作为氧探针以及黄色发射器作为内置参比探针组成，后者对葡萄糖或氧均无响应。所有这三个探针都通过化学方法固定在聚丙烯酰胺基质中。因此，该双传感器具有三个完全分离的发射颜色，并且可以应用比率法来分析生物条件下的葡萄糖和氧浓度。该传感器被用于实时监测细菌细胞、大肠杆菌（E. coli）和枯草芽孢杆菌（B. subtilis）以及鼠巨噬细胞 J774 和人宫颈癌 HeLa 细胞系的葡萄糖和氧消耗。另一方面，为了获得令人满意的葡萄糖感应性能，对作为葡萄糖探针接枝的连接聚合物的聚（2-羟乙基甲基丙烯酸酯）、聚丙烯酰胺和聚（6-氨基己基甲基丙烯酰胺）的基质组成进行了优化。
