Daly J A, Gooch W M, Matsen J M
J Clin Microbiol. 1985 Jun;21(6):919-21. doi: 10.1128/jcm.21.6.919-921.1985.
Meningitis of bacterial origin is a severe infection that must receive immediate attention and prompt treatment. We evaluated a basic fuchsin-methylene blue, ethyl alcohol-phenol staining procedure (Wayson stain) and compared it with the Gram stain procedure for evaluation of cerebrospinal fluid. All smears were prepared within 30 min of receiving the specimen and examined without knowledge of the results of the companion smear or culture. Of 546 specimens entered into the study, 84 were culture positive. Based on the culture results, the sensitivity and specificity of Wayson stain were 90 and 98%, respectively, compared with 73% sensitivity and 99% specificity by Gram stain. We observed that Wayson staining is a particularly sensitive method for screening clinical specimens that contain proteinaceous background material. The Wayson staining procedure is a simple and sensitive technique for early detection of meningitis.
细菌性脑膜炎是一种严重感染，必须立即引起关注并进行及时治疗。我们评估了一种碱性品红-亚甲蓝、乙醇-苯酚染色方法（韦逊染色法），并将其与革兰氏染色法用于脑脊液评估进行比较。所有涂片均在收到标本后30分钟内制备，并在不知道配对涂片或培养结果的情况下进行检查。在纳入该研究的546份标本中，84份培养呈阳性。根据培养结果，韦逊染色法的敏感性和特异性分别为90%和98%，而革兰氏染色法的敏感性为73%，特异性为99%。我们观察到，韦逊染色是筛查含有蛋白质背景物质的临床标本的一种特别敏感的方法。韦逊染色程序是早期检测脑膜炎的一种简单且敏感的技术。
