从脑脊液中分离出致病细菌。
Recovery of pathogenic bacteria from cerebrospinal fluid.
作者信息
Murray P R, Hampton C M
出版信息
J Clin Microbiol. 1980 Oct;12(4):554-7. doi: 10.1128/jcm.12.4.554-557.1980.
Abstract
We studied the conditions necessary for optimal recovery of bacteria from cerebrospinal fluid. Our results indicated that Streptopcoccus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae can be quantitatively recovered in the sediment after centrifugation at 1,500 X g for 15 min. Equivalent numbers of bacteria were recovered by centrifugation or filtration of antibiotic-free cerebrospinal fluid; however, bacterial recovery by filtration was less effective with antibiotic-supplemented cerebrospinal fluid.
摘要
我们研究了从脑脊液中最佳回收细菌所需的条件。我们的结果表明，肺炎链球菌、脑膜炎奈瑟菌和流感嗜血杆菌在1500×g离心15分钟后，可在沉淀物中定量回收。通过对无抗生素的脑脊液进行离心或过滤可回收等量的细菌；然而，对于添加抗生素的脑脊液，通过过滤回收细菌的效果较差。
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本文引用的文献
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Am J Clin Pathol. 1968 Mar;49(3):410-3. doi: 10.1093/ajcp/49.3.410.
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Acute bacterial meningitis: an analysis of factors influencing prognosis.
Am J Med Sci. 1975 Nov-Dec;270(3):427-40. doi: 10.1097/00000441-197511000-00003.
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Binding of aminoglycoside antibiotics to filtration materials.氨基糖苷类抗生素与过滤材料的结合。
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Relation of concentrations of bacteria and bacterial antigen in cerebrospinal fluid to prognosis in patients with bacterial meningitis.细菌性脑膜炎患者脑脊液中细菌及细菌抗原浓度与预后的关系。
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