Shanholtzer C J, Schaper P J, Peterson L R
J Clin Microbiol. 1982 Dec;16(6):1052-6. doi: 10.1128/jcm.16.6.1052-1056.1982.
A Cytospin slide centrifuge was used to concentrate 0.05- to 0.5-ml samples of cerebrospinal and other body fluids for Gram stain. Trials with cerebrospinal fluid containing known numbers of microorganisms indicated that the Cytospin increased the sensitivity of cerebrospinal fluid Gram stains by up to 2 logs compared with unconcentrated and conventional centrifuge smears. Cytospin-concentrated smears were prospectively compared with unconcentrated Gram-stained smears and bacteriological culture results for 80 clinical body fluid specimens. Bacteria were seen in unconcentrated smears of 9 of the 16 (56%) fluids which were infected, whereas Cytospin smears of 12 of the 16 (75%) showed bacteria. Cytospin smears revealed more bacteria and demonstrated better leukocyte morphology than did unconcentrated or conventionally centrifuged samples of small volumes of infected body fluids, allowing early diagnosis of infection.
使用细胞涂片离心机对0.05至0.5毫升的脑脊液和其他体液样本进行浓缩，以进行革兰氏染色。对含有已知数量微生物的脑脊液进行的试验表明，与未浓缩和传统离心涂片相比，细胞涂片可将脑脊液革兰氏染色的灵敏度提高多达2个对数。前瞻性地比较了细胞涂片浓缩涂片与80份临床体液标本的未浓缩革兰氏染色涂片及细菌培养结果。在16份受感染体液中的9份（56%）的未浓缩涂片中可见细菌，而在16份中的12份（75%）的细胞涂片中有细菌。与未浓缩或传统离心的少量受感染体液样本相比，细胞涂片显示出更多细菌，且白细胞形态更好，有助于早期诊断感染。
