血清基质金属蛋白酶-9 和组织金属蛋白酶抑制剂-1 水平在蜱传脑炎患者中的变化。
Serum matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 levels in patients with tick-borne encephalitis.
机构信息
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic; Faculty of Science, University of South Bohemia, Branišovská 31, CZ-37005 České Budějovice, Czech Republic.
Department of Virology, Hospital České Budějovice, Boženy Němcové 585/54, CZ-37001 České Budějovice, Czech Republic.
出版信息
J Infect. 2014 Feb;68(2):165-9. doi: 10.1016/j.jinf.2013.09.028. Epub 2013 Oct 1.
OBJECTIVES
Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) play important roles in the function of the blood-brain barrier (BBB). To investigate the function of the BBB during tick-borne encephalitis (TBE), the levels of MMP-9 and its common tissue inhibitor, TIMP-1, were measured in serum from patients with acute phase of TBE.
METHODS
Serum MMP-9 and TIMP-1 levels were measured in 147 patients with TBE and 153 controls by ELISA.
RESULTS
Serum MMP-9 levels and MMP-9/TIMP-1 ratios of TBE patients were significantly higher than controls (p < 0.0001 and p < 0.005, respectively). There were no significant differences in serum TIMP-1 levels between TBE patients and controls. Serum MMP-9 and TIMP-1 levels and MMP-9/TIMP-1 ratios were not associated with age of the patients. However, TBE-positive males with TBE had higher levels of MMP-9 than TBE-positive females (p < 0.05).
CONCLUSIONS
Our results suggest that the increased serum level of MMP-9 and MMP-9/TIMP-1 ratio is associated with the pathogenesis of TBE. Serum MMP-9 can serve as an indicator of breakdown of the BBB and inflammatory brain damage during TBE.
目的
基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制剂-1（TIMP-1）在血脑屏障（BBB）功能中发挥重要作用。为了研究蜱传脑炎（TBE）期间 BBB 的功能，通过 ELISA 法测量了急性 TBE 患者血清中 MMP-9 及其常见组织抑制剂 TIMP-1 的水平。
方法
共检测了 147 例 TBE 患者和 153 例对照者的血清 MMP-9 和 TIMP-1 水平。
结果
TBE 患者血清 MMP-9 水平和 MMP-9/TIMP-1 比值显著高于对照组（p<0.0001 和 p<0.005）。TBE 患者和对照组血清 TIMP-1 水平无显著差异。血清 MMP-9 和 TIMP-1 水平及 MMP-9/TIMP-1 比值与患者年龄无关。然而，TBE 阳性男性的 MMP-9 水平高于 TBE 阳性女性（p<0.05）。
结论
我们的结果表明，血清 MMP-9 水平升高和 MMP-9/TIMP-1 比值增加与 TBE 的发病机制有关。血清 MMP-9 可作为 TBE 期间 BBB 破坏和炎症性脑损伤的标志物。
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