Hajská Marianna, Slobodníková Lívia, Hupková Helena, Koller Ján
Teaching Department of Burns and Reconstructive Surgery, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia.
Institute of Microbiology, Medical Faculty of Comenius University and University Hospital, Bratislava, Slovakia.
Burns. 2014 Jun;40(4):713-8. doi: 10.1016/j.burns.2013.09.003. Epub 2013 Oct 3.
In vitro efficacy evaluation of eleven topical antimicrobials against multidrug-resistant (MDR) bacteria isolated from burn wounds of our patients.
Growth of six MDR bacterial strains: Pseudomonas aeruginosa (2 strains), Staphylococcus aureus, Staphylococcus haemolyticus, Enterococcus faecalis and Escherichia coli in burn-wound models was evaluated 24-h after application of the tested agents. Four different wound models were created to investigate the role of time elapsed between inoculation of bacteria and application of the agents on their antimicrobial activity and efficacy.
The efficacy against all the 6 bacteria in freshly contaminated wounds was excellent in majority of the tested agents. The longer was the time interval between inoculation and application of the topical antimicrobial agents, the higher failure of the agents was observed.
Topical antimicrobials play an important role in treatment of burn wounds, but they should be used according to their efficacy against bacterial strains present in patients' wounds. In cases where topical agents have been applied after 24 h, when formation of mature biofilm in the wound could be expected, it would probably not be possible to kill all the bacteria using topical antimicrobial therapy only.
对从我院患者烧伤创面分离出的耐多药（MDR）细菌，评估11种局部用抗菌药物的体外疗效。
在烧伤创面模型中，应用受试药物24小时后，评估6种耐多药菌株的生长情况，这些菌株包括铜绿假单胞菌（2株）、金黄色葡萄球菌、溶血葡萄球菌、粪肠球菌和大肠杆菌。创建了4种不同的创面模型，以研究细菌接种与药物应用之间的时间间隔对其抗菌活性和疗效的影响。
在大多数受试药物中，对新鲜污染创面中所有6种细菌的疗效都非常好。局部用抗菌药物接种与应用之间的时间间隔越长，观察到的药物失效情况就越高。
局部用抗菌药物在烧伤创面治疗中起着重要作用，但应根据其对患者创面中存在的细菌菌株的疗效来使用。在24小时后应用局部用药的情况下，此时创面可能会形成成熟的生物膜，仅使用局部抗菌治疗可能无法杀死所有细菌。
