Van Dongen J J, Hooijkaas H, Comans-Bitter W M, Benne K, Van Os T M, De Josselin de Jong J
J Immunol Methods. 1985 Jun 12;80(1):1-6. doi: 10.1016/0022-1759(85)90157-7.
Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers. We analyzed human bone marrow cells by a triple immunological staining for 3 different markers, using colloidal gold, fluorescein and rhodamine as labels. Our results demonstrate that such a triple immunological staining provides a powerful tool for study of the heterogeneity of small cell populations.
在与荧光显微镜相同的落射照明系统中，用偏光通过光学显微镜可检测到胶体金颗粒。因此，胶体金颗粒可与荧光染料结合使用，用于表面膜和细胞质标志物的联合免疫检测。我们使用胶体金、荧光素和罗丹明作为标记物，通过对3种不同标志物进行三重免疫染色来分析人骨髓细胞。我们的结果表明，这种三重免疫染色为研究小细胞群体的异质性提供了一个强大的工具。
