Hapner S J, Hapner K D
J Histochem Cytochem. 1978 Jun;26(6):478-82. doi: 10.1177/26.6.353187.
Tissue slices from the roots and seeds of sanifoin (Onobrychis viciifolia, Scop.) exhibit bright autofluorescence when illuminated with blue (495 nm) light. This autofluorescence is indistinguishable from the fluorescence emission of fluorescein, the commonly used fluorochrome in immunohistochemical staining procedures. Rhodamine isothiocyanate, when coupled to immunoglobulin, and excited with green light at 546 nm, exhibits a reddish-orange fluorescence with an emission maximum at 590 nm. Plant tissue has little or no autofluorescence when illuminated at this wavelength and viewed with a 580 nm barrier filter. Therefore, use of rhodamine for immunohistochemical localization in plant tissue avoids interpretative complications due to inherent autofluorescence.
红豆草（Onobrychis viciifolia, Scop.）根和种子的组织切片在蓝光（495纳米）照射下呈现明亮的自发荧光。这种自发荧光与免疫组织化学染色程序中常用的荧光染料荧光素的荧光发射无法区分。异硫氰酸罗丹明与免疫球蛋白偶联后，在546纳米的绿光激发下，呈现出红橙色荧光，发射最大值在590纳米。在这个波长下照射并使用580纳米的阻挡滤光片观察时，植物组织几乎没有自发荧光。因此，在植物组织中使用罗丹明进行免疫组织化学定位可避免因固有自发荧光而产生的解释复杂性。
