Allner R
J Clin Chem Clin Biochem. 1985 Apr;23(4):231-40.
Fibrinogen interferes in heterogeneous enzyme immunological in vitro tests. The effect on the test result is variable and depends on the test principle (competitive binding analysis or sandwich technique). In the tests for digoxin, cortisol, alpha 1-fetoprotein and acid prostate phosphatase, the use of plasma as sample material resulted in varying degrees of interference. In the case of the digoxin determination, using the Enzymun-Test Digoxin, it is possible to abolish interference by fibrinogen by thermal coagulation (plasma is heated at 56 degrees C for 30 min) and removal of the fibrinogen. Alternatively, addition of urea to the antigen-antibody reaction mixture decreases the interference caused by fibrinogen. The added quantity of urea and the time of incubation must be optimized for each sample material.
纤维蛋白原会干扰体外异相酶免疫试验。对检测结果的影响是可变的，且取决于检测原理（竞争性结合分析或夹心技术）。在洋地黄毒苷、皮质醇、甲胎蛋白和酸性前列腺磷酸酶的检测中，使用血浆作为样本材料会产生不同程度的干扰。在使用酶免疫法检测洋地黄毒苷时，通过热凝固法（将血浆在56℃加热30分钟）去除纤维蛋白原，有可能消除纤维蛋白原的干扰。或者，向抗原 - 抗体反应混合物中添加尿素可减少纤维蛋白原引起的干扰。添加尿素的量和孵育时间必须针对每种样本材料进行优化。
