Specific detection of mycobacteria by the immunofluorescent technique.

Using an indirect immunofluorescent technique, M. bovis var. BCG can be differentiated from M. avium in pure culture. The test involves using an unlabeled antiserum prepared against B-24, a type specific antigen of M. bovis var. BCG. In the same system M. bovis could be differentiated from other pathogenic my cobacteria in tissue sections of naturally and experimentally infected animals.