Department of Molecular Biotechnology, College of Life and Environmental Sciences, Konkuk University , Seoul , South Korea.
Pharm Biol. 2014 Feb;52(2):221-7. doi: 10.3109/13880209.2013.831110. Epub 2013 Oct 4.
Oxidative stress and inflammation are related to several chronic diseases including cancer and atherosclerosis. Arisaema tortuosum (Wall.) Schott (Araceae) is an Indian folk medicinal herb traditionally used for treatment of various diseases related to inflammation and stress.
This study was carried out for HPTLC analysis and evaluation of antioxidant, anti-inflammatory and antiproliferative activities of a methanol extract of A. tortuosum tuber.
The antioxidant activities of methanol extract of A. tortuosum tuber (1 mg/mL) were evaluated by DPPH, ABTS and FRAP assays and anti-inflammatory effects by diene-conjugate and β-glucuronidase assays, with in vitro tumor growth inhibition on HeLa cancer cells. The results for antioxidant and anti-inflammatory effects were compared using Trolox and salicylic acid as reference compounds, respectively.
The TLC and HPTLC analysis showed the presence of quercetin, rutin, luteolin and lectin (Rf values 0.97, 0.53, 0.59 and 1.58, respectively). The methanol fraction of tuber exhibit higher activity in each antioxidant system with a special attention for DPPH (IC50 = 852 μg/mL), ABTS (IC50 = 532 μg/mL), and FRAP (IC50 = 458 μg/mL), as compared with Trolox as standard, with a remarkable amount of phenolics (86.2 mg/100 g) and flavonoids (175.5 mg/100 g), along with potent anti-inflammatory activity indicated by diene-conjugate (86.20%) and β-glucuronidase (92.92%) inhibition, as compared with salicylic acid as reference compound. The antiproliferative activity at 100 mg/mL was 88% inhibition with HeLa cells. The inhibition of HeLa cell proliferation was greatest (p < 0.001) with the 100 mg/mL A. tortuosum tuber extract treatments and least with the 25 mg/mL dose.
Our results suggested that A. tortuosum tuber might be used as a promising and potent antioxidant, anti-inflammatory, and antiproliferative agent and might be used for standardization of potential drug after successful isolation and characterization of bioactive compounds.
氧化应激和炎症与多种慢性疾病有关，包括癌症和动脉粥样硬化。天南星（天南星科）是一种印度民间药用植物，传统上用于治疗与炎症和应激有关的各种疾病。
本研究进行了高效薄层色谱分析，并评估了天南星块茎甲醇提取物的抗氧化、抗炎和抗增殖活性。
采用 DPPH、ABTS 和 FRAP 测定法评价天南星块茎甲醇提取物（1mg/mL）的抗氧化活性，并通过体外肿瘤生长抑制试验评价其抗炎作用在 HeLa 癌细胞上。抗氧化和抗炎作用的结果分别用 Trolox 和水杨酸作为参考化合物进行比较。
TLC 和 HPTLC 分析表明，槲皮素、芦丁、木犀草素和凝集素（Rf 值分别为 0.97、0.53、0.59 和 1.58）存在。与 Trolox 作为标准相比，块茎的甲醇部分在每个抗氧化系统中表现出更高的活性，特别是对 DPPH（IC50=852μg/mL）、ABTS（IC50=532μg/mL）和 FRAP（IC50=458μg/mL），同时还含有大量的酚类（86.2mg/100g）和类黄酮（175.5mg/100g），以及与水杨酸作为参考化合物相比，对二烯共轭（86.20%）和β-葡萄糖醛酸酶（92.92%）的抑制作用具有显著的抗炎活性。在 100mg/mL 时，对 HeLa 细胞的增殖抑制率为 88%。用 100mg/mL 的天南星块茎提取物处理时，对 HeLa 细胞增殖的抑制作用最大（p<0.001），而用 25mg/mL 剂量时抑制作用最小。
我们的结果表明，天南星块茎可能被用作一种有前途的、有效的抗氧化剂、抗炎剂和抗增殖剂，并可能在成功分离和表征生物活性化合物后，用于潜在药物的标准化。
