Molecular Ecology Group, State Key Laboratory of Grassland Agro-ecosystem, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, China.
BMC Plant Biol. 2013 Oct 5;13:152. doi: 10.1186/1471-2229-13-152.
Corylus was renowned for its production of hazelnut and taxol. To understand the local adaptation of Chinese species and speed up breeding efforts in China, we analyzed the leaf transcriptome of Corylus mandshurica, which had a high tolerance to fungal infections and cold.
A total of 12,255,030 clean pair-end reads were generated and then assembled into 37,846 Expressed Sequence Tag (EST) sequences. During functional annotation, 26,565 ESTs were annotated with Gene Ontology (GO) terms using Blast2go and 11,056 ESTs were grouped into the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways using KEGG Automatic Annotation Server (KAAS). We identified 45 ESTs that were homologous to enzymes and transcription factors responsible for taxol synthesis. The most differentiated orthologs between C. mandshurica and a European congener, C. avellana, were enriched in stress tolerance to fungal resistance and cold.
In this study, we detected a set of genes related to taxol synthesis in a taxol-producing angiosperm species for the first time and found a close relationship between most differentiated genes and different adaptations to fungal infection and cold in C. mandshurica and C. avellana. These findings provided tools to improve our understanding of local adaptation, genetic breeding and taxol production in hazelnut.
榛属以生产榛子和紫杉醇而闻名。为了了解中国物种的本地适应性并加速中国的育种工作，我们分析了具有高抗真菌感染和抗寒能力的毛榛的叶片转录组。
共生成了 12255030 对清洁的双端读取片段，然后组装成 37846 条表达序列标签（EST）序列。在功能注释过程中，使用 Blast2go 将 26565 条 ESTs 注释到基因本体论（GO）术语中，使用 KEGG 自动注释服务器（KAAS）将 11056 条 ESTs 分组到京都基因与基因组百科全书（KEGG）途径中。我们鉴定了 45 条与紫杉醇合成相关的酶和转录因子的 ESTs。毛榛与欧洲同类种榛子之间最具差异的直系同源物在抗真菌和抗寒方面的耐受力方面得到了富集。
在这项研究中，我们首次在产紫杉醇的被子植物物种中检测到一组与紫杉醇合成相关的基因，并发现毛榛和榛子之间大多数分化基因与抗真菌感染和抗寒能力的不同适应之间存在密切关系。这些发现为我们理解榛子的本地适应性、遗传育种和紫杉醇生产提供了工具。
