Division of Biochemistry, Department of Chemistry, Hacettepe University , Ankara , Turkey.
Artif Cells Nanomed Biotechnol. 2015 Feb;43(1):62-70. doi: 10.3109/21691401.2013.841172. Epub 2013 Oct 4.
The selective binding of albumin on dye-affinity nanospheres was combined with magnetic properties as an alternative approach for albumin depletion from human plasma. Magnetic poly(hydroxyethyl methacrylate) (mPHEMA) nanospheres were synthesized using mini-emulsion polymerization method in the presence of magnetite powder. The specific surface area of the mPHEMA nanospheres was found to be 1302 m(2)/g. Subsequent to Cibacron Blue F3GA (CB) immobilization onto mPHEMA nanospheres, a serial characterization processing was implemented. The quantity of immobilized CB was calculated as 800 μmol/g. Ultimately, albumin adsorption performance of the CB-attached mPHEMA nanospheres from both aqueous dissolving medium and human plasma were explored.
将染料亲和纳米球上的白蛋白选择性结合，并结合磁性，作为从人血浆中去除白蛋白的一种替代方法。采用细乳液聚合方法，在磁铁矿粉末存在的条件下合成了磁性聚（羟乙基甲基丙烯酸酯）（mPHEMA）纳米球。发现 mPHEMA 纳米球的比表面积为 1302 m2/g。在将 Cibacron Blue F3GA（CB）固定到 mPHEMA 纳米球上之后，进行了一系列的特性分析处理。固定化 CB 的量计算为 800 μmol/g。最后，研究了 CB 修饰的 mPHEMA 纳米球在水溶解介质和人血浆中的白蛋白吸附性能。
