Arica M Y, Testereci H N, Denizli A
Department of Biology, Kirikkale University, Turkey.
J Chromatogr A. 1998 Mar 13;799(1-2):83-91. doi: 10.1016/s0021-9673(97)01079-0.
Cibacron Blue F3GA was covalently immobilized onto poly(2-hydroxyethyl methacrylate) pHEMA) membranes via the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl group of pHEMA. Then, Fe3+ ions were complexed by chelation with the immobilized Cibacron Blue F3GA molecules. Different amounts of Fe3+ ions were loaded on the membranes by changing the concentration of Fe3+ ions and pH of the reaction medium. Membranes with or without Fe3+ were used in the adsorption of glucose oxidase, catalase and bovine serum albumin. The adsorption capacities of these membranes were determined by changing pH and the concentration of the proteins in the adsorption medium. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum capacities (qm) of the Fe3+ complexed membranes for glucose oxidase, catalase and bovine serum albumin (8.70 x 10(-3) mumol m-2, 2.15 x 10(-3) mumol m-2 and 2.21 x 10(-3) mumol m-2) were greater than those of the untreated membranes (6.79 x 10(-3) mumol m-2, 1.34 x 10(-3) mumol m-2 and 1.94 x 10(-3) mumol m-2) respectively. The nonspecific adsorption of the enzymes and the protein on the pHEMA membranes was negligible.
通过其三嗪环的氯与聚甲基丙烯酸2-羟乙酯（pHEMA）的羟基之间的亲核反应，将汽巴克隆蓝F3GA共价固定在pHEMA膜上。然后，通过与固定化的汽巴克隆蓝F3GA分子螯合使Fe3+离子络合。通过改变Fe3+离子的浓度和反应介质的pH值，将不同量的Fe3+离子负载到膜上。使用含或不含Fe3+的膜吸附葡萄糖氧化酶、过氧化氢酶和牛血清白蛋白。通过改变吸附介质的pH值和蛋白质浓度来测定这些膜的吸附容量。吸附现象似乎遵循典型的朗缪尔等温线。Fe3+络合膜对葡萄糖氧化酶、过氧化氢酶和牛血清白蛋白的最大吸附容量（qm）（分别为8.70×10(-3) μmol m-2、2.15×10(-3) μmol m-2和2.21×10(-3) μmol m-2）大于未处理膜（分别为6.79×10(-3) μmol m-2、1.34×10(-3) μmol m-2和1.94×10(-3) μmol m-2）。酶和蛋白质在pHEMA膜上的非特异性吸附可忽略不计。
