Sreejith M, Kannappan N, Santhiagu A, Mathew Ajith P
Department of Pharmaceutical Chemistry, National College of Pharmacy, Calicut, Kerala.
Asian Pac J Trop Biomed. 2013 Dec;3(12):947-53. doi: 10.1016/S2221-1691(13)60184-7.
The present study was carried out to investigate the phytochemical constituents, In vitro antioxidant potential and anthelmintic activities of Flacourtia sepiaria Roxb leaves.
The dried powdered leaves of Flacourtia sepiaria were extracted using petroleum ether, chloroform, ethyl acetate and methanol by a soxhlet extractor and preliminary phytochemical screening was performed using standard protocols. All the extract was evaluated for their potential antioxidant activities using test such as DPPH, superoxide anion radical, hydroxyl radical, nitric oxide radical scavenging abilities, ferrous chelating ability and total phenolic and flavanoid content. Anthelmintic activity of extract was screened in adult Indian earthworm model.
Preliminary screening revealed the presence of bioactive compounds especially phenolics, tannins and terpenoids in all extracts. The phenolic and flavanoid content was highest in methanolic extract and lowest in petroleum ether extract. The paralytic (9.46±0.212) and death time (31.43±0.148) of methanolic extract was found to be significant (P<0.05) when compared with paralytic (7.33±0.206) and death time (18.60±0.229) of standard piperazine citrate at 100 mg/mL concentration.
The results of the present study indicate that the leaf extracts of Flacourtia sepiaria exhibited strong antioxidant activity and possess significant anthelmintic activity and thus it is a good source of antioxidant and anthelmintic constituents.
本研究旨在调查隔距叶大风子（Flacourtia sepiaria Roxb）叶的植物化学成分、体外抗氧化潜力及驱虫活性。
采用索氏提取器，用石油醚、氯仿、乙酸乙酯和甲醇对隔距叶大风子的干燥粉末状叶子进行提取，并使用标准方法进行初步植物化学筛选。使用DPPH、超氧阴离子自由基、羟基自由基、一氧化氮自由基清除能力、亚铁螯合能力以及总酚和类黄酮含量等测试评估所有提取物的潜在抗氧化活性。在成年印度蚯蚓模型中筛选提取物的驱虫活性。
初步筛选显示所有提取物中均存在生物活性化合物，尤其是酚类、单宁和萜类化合物。甲醇提取物中的酚类和类黄酮含量最高，石油醚提取物中的含量最低。与100 mg/mL浓度的标准枸橼酸哌嗪的麻痹时间（7.33±0.206）和死亡时间（18.60±0.229）相比，甲醇提取物的麻痹时间（9.46±0.212）和死亡时间（31.43±0.148）具有显著性差异（P<0.05）。
本研究结果表明，隔距叶大风子叶提取物具有较强的抗氧化活性和显著的驱虫活性，因此是抗氧化剂和驱虫成分的良好来源。
