Shoibe Mohammed, Chy Md Nazim Uddin, Alam Morshed, Adnan Md, Islam Md Zobidul, Nihar Shababa Wajida, Rahman Nishat, Suez Ehsan
Pharmacognosy and Phytochemistry Lab, Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh.
Drug Discovery, GUSTO A Research Group, Chittagong 4000, Bangladesh.
Biomedicines. 2017 Oct 30;5(4):63. doi: 10.3390/biomedicines5040063.
This study was conducted to evaluate the in vitro polyphenol content, antioxidant, cytotoxic, antibacterial, anthelmintic properties, and in vivo antinociceptive activity of the ethanol extract of leaves (EECA) in different experimental models. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) radical-scavenging, ferric reducing power, and total antioxidant capacity assays. Cytotoxicity was determined by brine shrimp lethality bioassay and disc diffusion method was used for the antibacterial activity. Anthelmintic activity was studied using aquarium worm () whereas antinociceptive activity was evaluated in mice by acetic acid and formalin test. Phytochemical screening of EECA revealed the presence of alkaloids, carbohydrates, flavonoids, phenols, terpenoids, saponins, and tannins. EECA showed strong antioxidant activity with high polyphenol contents. It was observed that EECA possessed significant antibacterial activity with a low toxicity profile. EECA also demonstrated dose-dependent and statistically significant anthelmintic and antinociceptive activities. Our study shows that ethanol extract of leaves possess strong antioxidant, antibacterial, anthelmintic and antinociceptive activities with lower toxicity. Further studies are needed to identify bioactive phytomolecules and to understand the mechanism of such actions better.
本研究旨在评估不同实验模型中叶乙醇提取物（EECA）的体外多酚含量、抗氧化、细胞毒性、抗菌、驱虫特性以及体内抗伤害感受活性。采用分光光度技术研究多酚含量。通过1,1-二苯基-2-苦基肼自由基（DPPH）自由基清除、铁还原能力和总抗氧化能力测定来确定抗氧化活性。通过卤虫致死生物测定法确定细胞毒性，并采用纸片扩散法测定抗菌活性。使用水族箱蠕虫研究驱虫活性，而通过乙酸和福尔马林试验在小鼠中评估抗伤害感受活性。EECA的植物化学筛选显示存在生物碱、碳水化合物、黄酮类、酚类、萜类、皂苷和单宁。EECA表现出具有高多酚含量的强抗氧化活性。观察到EECA具有显著的抗菌活性且毒性较低。EECA还表现出剂量依赖性且具有统计学意义的驱虫和抗伤害感受活性。我们的研究表明，叶乙醇提取物具有较强的抗氧化、抗菌、驱虫和抗伤害感受活性且毒性较低。需要进一步研究以鉴定生物活性植物分子并更好地理解此类作用的机制。
