Department of Infectious Diseases and Microbiology, University of Pittsburgh, United States.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4575-81. doi: 10.1016/j.msec.2013.07.013. Epub 2013 Jul 20.
Environmental mycobacteria pose a significant health burden. Non-tuberculous mycobacteria infections have been traced to water treatment networks, where mycobacterial biofilms are ubiquitous. Filters that remove potential pathogens have significant medical applications. The purpose of this study is to demonstrate that an antibacterial silver nanoparticle (AgNP) coating can prevent colonization and growth of a mycobacterial biofilm on a filter material. The antibacterial efficacy of commercially available AgNPs was measured against Mycobacterium avium, Mycobacterium smegmatis, and Mycobacterium marinum after 48 h in liquid culture. Nanoparticles were deposited on micro-porous track etched polycarbonate membranes. The growth of biofilms on the membranes was observed by microscopy and counting colony forming units. M. smegmatis was most susceptible to AgNPs, with a 98.7% reduction at 100 μM AgNP concentration. M. avium was reduced by 97.3% at 539 μM AgNP after 48 h. Deposited nanoparticles inhibited colonization and growth for both M. smegmatis and M. avium on the membrane surface. Similar to the liquid culture, M. avium (84.2% survival) was more resistant than M. smegmatis (0.03% survival).
环境分枝杆菌构成重大健康负担。非结核分枝杆菌感染已被追踪到水疗网络，分枝杆菌生物膜无处不在。去除潜在病原体的过滤器具有重要的医学应用。本研究旨在证明抗菌银纳米粒子（AgNP）涂层可以防止分枝杆菌生物膜在过滤材料上的定植和生长。在液体培养 48 小时后，测量了市售 AgNPs 对鸟分枝杆菌、耻垢分枝杆菌和海分枝杆菌的抗菌功效。将纳米颗粒沉积在微孔径轨迹蚀刻聚碳酸酯膜上。通过显微镜观察和菌落形成单位计数来观察生物膜在膜上的生长。耻垢分枝杆菌对 AgNPs 最敏感，在 100 μM AgNP 浓度下减少 98.7%。48 小时后，539 μM AgNP 使鸟分枝杆菌减少 97.3%。沉积的纳米颗粒抑制了膜表面上耻垢分枝杆菌和鸟分枝杆菌的定植和生长。与液体培养相似，鸟分枝杆菌（84.2%存活）比耻垢分枝杆菌（0.03%存活）更具抗性。
