Department of Chemical and Food Engineering, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4684-91. doi: 10.1016/j.msec.2013.07.029. Epub 2013 Jul 26.
When cultured under static conditions, bacterial cellulose pellicles, by the nature of the polymer synthesis that involves molecular oxygen, are characterized by two distinct surface sides. The upper surface is denser in fibers (entangled) than the lower surface that shows greater surface porosity. Human umbilical vein endothelial cells (HUVECs) were used to exploit how the microarchitecture (i.e., surface porosity, fiber network structure, surface topology, and fiber density) of bacterial cellulose pellicle surfaces influence cell-biomaterial interaction and therefore cell behavior. Adhesion, cell ingrowth, proliferation, viability and cell death mechanisms were evaluated on the two pellicle surface sides. Cell behavior, including secondary necrosis, is influenced only by the microarchitecture of the surface, since the biomaterial is extremely pure (constituted of cellulose and water only). Cell-cellulose fiber interaction is the determinant signal in the cell-biomaterial responses, isolated from other frequently present interferences such as protein and other chemical traces usually present in cell culture matrices. Our results suggest that microarchitecture of hydrogel materials might determine the performance of biomedical products, such as bacterial cellulose tissue engineering constructs (BCTECs).
在静态条件下培养时，由于涉及分子氧的聚合物合成，细菌纤维素膜具有两个明显不同的表面。上表面的纤维（缠结）比下表面更密集，下表面显示出更大的表面孔隙率。本研究使用人脐静脉内皮细胞（HUVEC）来探索细菌纤维素膜表面的微观结构（即表面孔隙率、纤维网络结构、表面拓扑和纤维密度）如何影响细胞-生物材料相互作用，从而影响细胞行为。在上、下两个膜表面分别评估了细胞黏附、细胞内生长、增殖、活力和细胞死亡机制。细胞行为，包括继发性坏死，仅受表面微观结构的影响，因为生物材料非常纯净（仅由纤维素和水组成）。细胞-纤维素纤维相互作用是细胞-生物材料反应中的决定信号，可排除细胞培养基质中通常存在的其他干扰因素，如蛋白质和其他化学痕迹。我们的研究结果表明，水凝胶材料的微观结构可能决定生物医学产品的性能，如细菌纤维素组织工程构建体（BCTECs）。
