Department of Microtechnology and Nanoscience, Chalmers University of Technology, Kemivägen 9, SE-412 96 Gothenburg, Sweden; Wallenberg Wood Science Center, Chalmers University of Technology, Kemivägen 4, SE-412 96 Gothenburg, Sweden.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4599-607. doi: 10.1016/j.msec.2013.07.031. Epub 2013 Jul 30.
Bacterial nanocellulose (BNC) is an emerging biomaterial since it is biocompatible, integrates well with host tissue and can be biosynthesized in desired architecture. However, being a hydrogel, it exhibits low affinity for cell attachment, which is crucial for the cellular fate process. To increase cell attachment, the surface of BNC scaffolds was modified with two proteins, fibronectin and collagen type I, using an effective bioconjugation method applying 1-cyano-4-dimethylaminopyridinium (CDAP) tetrafluoroborate as the intermediate catalytic agent. The effect of CDAP treatment on cell adhesion to the BNC surface is shown for human umbilical vein endothelial cells and the mouse mesenchymal stem cell line C3H10T1/2. In both cases, the surface modification increased the number of cells attached to the surfaces. In addition, the morphology of the cells indicated more healthy and viable cells. CDAP activation of bacterial nanocellulose is shown to be a convenient method to conjugate extracellular proteins to the scaffold surfaces. CDAP treatment can be performed in a short period of time in an aqueous environment under heterogeneous and mild conditions preserving the nanofibrillar network of cellulose.
细菌纳米纤维素（BNC）是一种新兴的生物材料，因为它具有生物相容性，能与宿主组织很好地结合，并可按照所需结构进行生物合成。然而，作为一种水凝胶，它对细胞附着的亲和力较低，而细胞附着对于细胞命运过程至关重要。为了提高细胞附着性，使用有效的生物偶联方法，以 1-氰基-4-二甲基氨基吡啶四氟硼酸盐（CDAP）作为中间催化试剂，将两种蛋白质，纤连蛋白和 I 型胶原，修饰到 BNC 支架的表面。CDAP 处理对人脐静脉内皮细胞和小鼠间充质干细胞系 C3H10T1/2 细胞在 BNC 表面的粘附的影响。在这两种情况下，表面修饰都增加了附着在表面上的细胞数量。此外，细胞的形态表明细胞更健康和更有活力。研究表明，CDAP 激活细菌纳米纤维素是将细胞外蛋白偶联到支架表面的一种便捷方法。CDAP 处理可在水相环境中、非均相和温和条件下短时间内进行，从而保持纤维素的纳米纤维网络。
