Wollenberg P, Rummel W
Naunyn Schmiedebergs Arch Pharmacol. 1985 Apr;329(2):195-200. doi: 10.1007/BF00501212.
An in vitro vascularly and luminally perfused preparation of the murine small intestine was used to investigate the interference of isethionate, cyclamate and HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) with the luminal transport of the isoprenaline-sulfoconjugate as well as with the basolateral transport of naphthol-sulfoconjugate. The sulfonates and sulfamates when administered from the luminal as well as from the contraluminal side of the epithelium inhibited the transport of isoprenaline-sulfoconjugate. Inhibition of the naphthol-sulfoconjugate transport across the contraluminal epithelial membrane was less pronounced, but a countertransport phenomenon could be induced with cyclamate in the vascular medium. The presence of phosphate at the luminal side is essential for the transport of the isoprenaline-sulfoconjugate across the luminal membrane. This is not the case for bicarbonate. The conclusion is drawn that different transport systems for sulfoconjugates exist in the luminal and in the contraluminal membranes of the intestinal mucosa, which can be inhibited by structurally related compounds. The luminal transport system can be activated from the luminal side by phosphate.
使用小鼠小肠的体外血管内和管腔内灌注制剂，研究羟乙磺酸盐、甜蜜素和4-羟乙基哌嗪乙磺酸（HEPES）对异丙肾上腺素硫酸共轭物的管腔转运以及萘酚硫酸共轭物的基底外侧转运的干扰。当从上皮的管腔侧以及对侧管腔侧给予磺酸盐和氨基磺酸盐时，它们会抑制异丙肾上腺素硫酸共轭物的转运。萘酚硫酸共轭物跨对侧管腔上皮膜的转运抑制作用不太明显，但在血管介质中甜蜜素可诱导逆向转运现象。管腔侧存在磷酸盐对于异丙肾上腺素硫酸共轭物跨管腔膜的转运至关重要。碳酸氢盐则并非如此。得出的结论是，肠黏膜的管腔膜和对侧管腔膜中存在不同的硫酸共轭物转运系统，这些系统可被结构相关的化合物抑制。管腔转运系统可被管腔侧的磷酸盐激活。
