Wollenberg P, Ullrich V, Rummel W
Biochem Pharmacol. 1983 Jul 1;32(13):2103-7. doi: 10.1016/0006-2952(83)90433-1.
A method is described which allows the simultaneous vascular and luminal perfusion of the murine small intestine. This preparation was used for the investigation of 1-naphthol conjugation in the gut and the sidedness of conjugate release. The viability of this preparation can be maintained for more than 1 hr as indicated by morphological controls, measurement of tissue metabolism and the transport of 3-O-methyl-glucose against a concentration gradient. When 100 microM 1-naphthol was administered on the luminal side, it was conjugated at a constant rate, yielding 1-naphthyl-glucuronide and 1-naphthyl-sulfate in a molar ratio of 1:2. Both metabolites were excreted into the blood at the contraluminal side of the epithelium. The results are discussed with respect to the sidedness of intestinal transport systems for anionic conjugates of xenobiotics and drugs.
本文描述了一种可同时对小鼠小肠进行血管灌注和肠腔灌注的方法。该制备方法用于研究肠道中1-萘酚的结合作用以及结合物释放的方向性。形态学对照、组织代谢测量以及3-O-甲基葡萄糖逆浓度梯度转运结果表明，该制备物的活力可维持1小时以上。当在肠腔侧给予100微摩尔的1-萘酚时，它以恒定速率结合，生成摩尔比为1:2的1-萘基葡萄糖醛酸苷和1-萘基硫酸盐。两种代谢产物均在上皮细胞的对腔侧排入血液。针对外源性物质和药物的阴离子结合物的肠道转运系统的方向性对结果进行了讨论。
