Kochetkova G V, Matiushenkova E A, Ukholina R S, Kovsharova I N, Uspenskaia T A
Antibiotiki. 1975 Jun;20(6):499-502.
A biological method for determination of geliomycin activity using agar diffusion is described. A nutrient medium containing Hottinger broth up to 35 mg per cent of amine nitrogen, 1.5 percent of agar-agar, tap water, pH 7.8 to 8.0 was used. Bacillus subtilis ATCC 6633 served as the test-culture. Geliomycin was dissolved in 0.1 N sodium hydroxide solution.
描述了一种使用琼脂扩散法测定格利霉素活性的生物学方法。使用了一种营养培养基,其含有高达每百分35毫克胺氮的霍廷格肉汤、1.5%的琼脂、自来水,pH值为7.8至8.0。枯草芽孢杆菌ATCC 6633用作测试培养物。格利霉素溶解于0.1N氢氧化钠溶液中。