Wenyingzhuangia marina gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a recirculating mariculture system.

A Gram-stain-negative, strictly aerobic and heterotrophic bacterial strain, designed strain D1(T), was isolated from a recirculating mariculture system in Tianjin, China. Its taxonomic position was determined using a polyphasic approach. Cells of strain D1(T) were non-flagellated short rods, 0.3-0.5 µm wide and 0.5-1.0 µm long. Growth was observed at 15-30 °C (optimum, 25 °C), at pH 5.5-9.0 (optimum, pH 6.5-7.0) and in the presence of 1-8% (w/v) NaCl (optimum, 2-3 %). Cells contained carotenoid pigments but not flexirubin-type pigments. Strain D1(T) contained MK-6 as the sole menaquinone and phosphatidylethanolamine (PE) as the sole phospholipid and four unidentified lipids. The major cellular fatty acids (>10%) were iso-C15 : 0 (23.2 %), iso-C17 : 0 3-OH (15.2%), C(16 : 1)ω7c/C(16 : 1)ω6c (14.3%), iso-C(15 : 0) 3-OH (13.5%) and iso-C15 : 1 G (10.8%). 16S rRNA gene sequence analyses indicated that strain D1(T) belonged to the family Flavobacteriaceae and showed closest phylogenetic relationship to the genus Lutibacter, with highest sequence similarity to Lutibacter aestuarii MA-My1(T) (92.2%). The DNA G+C content of strain D1(T) was 35.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain D1(T) was considered to represent a novel species in a new genus of the family Flavobacteriaceae, for which the name Wenyingzhuangia marina gen. nov., sp. nov. is proposed. The type strain of the type species is D1(T) ( = CGMCC 1.12162(T) = JCM 18494(T)).