Draft Genome Sequence Analyses of Two Novel sp. nov. and sp. nov. Isolated from the Root of Makino.

Gram-negative, rod-shaped, and aerobic bacteria designated chi1 and chi5 were isolated from the root of Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1 and chi5 confirmed that they were related to the genera and , respectively. For the novel strains chi1 and chi5, the digital DNA-DNA hybridization values (19-20% and 22.1-36.6%, respectively) and average nucleotide identity values (74.4-76.5% and 79.1-88.9%, respectively) fell within the range for the genera and , respectively. Pangenome analyses of the novel strains chi1 and chi5 revealed 357 and 368 singletons genes, respectively. The genomic DNA G + C contents of the strains chi1 and chi5 were 57.2% and 31.5%, respectively. The major fatty acids of strain chi1 were C, C, and summed feature 3 (C6 and/or C7), while those of the strain chi5 were iso-C 3OH, iso-C 3OH, and iso-C. Data from the phylogenetic, phylogenomic, pangenome, genomic, physiological, and biochemical analyses indicated that the novel strains were distinct. Therefore, we propose the names (type strain chi1 = KACC 23259 = TBRC 17652) and (type strain chi5 = KACC 23262 = TBRC 17900) for the studied bacterial strains.