Schumacher J M, Eroschenko V P
Toxicology. 1985 Jun 14;35(3):219-30. doi: 10.1016/0300-483x(85)90017-4.
To investigate the effects of the estrogenic insecticide chlordecone on the morphology of mouse choroid plexus, 4 different doses (100.0, 250.0, 500.0 and 1000.0 micrograms) of the chemical were administered intraperitoneally to groups of adult males. Concurrent with the chlordecone treatments, another group of males received 10.0 micrograms estradiol-17 beta; the control group was treated with sesame oil vehicle. After 15 days of daily chemical injections, the mice were terminated and the choroid plexus from fourth ventricle examined morphologically. Scanning (SEM) and transmission (TEM) electron microscopic examination of control choroid plexus showed the cuboidal epithelium profusely covered with microvilli. SEM examinations of choroid plexus epithelium after chlordecone treatments revealed dose-dependent, cell alterations that effected the microvilli and cell surfaces. After the highest chlordecone dose, microvilli were no longer visible, the choroidal cell membrane appeared either smooth or pitted and there was evidence of increased luminal debris. TEM observations of the same choroid plexus cells revealed vacuolated cytoplasm, dilated endoplasmic reticulum, vacuolated mitochondria with disrupted cristae and cellular degeneration. SEM and TEM examination of choroid plexus after estradiol treatments revealed similar cellular alterations to those recorded after chlordecone treatments. The possible significance of these data are discussed.
为研究雌激素类杀虫剂十氯酮对小鼠脉络丛形态的影响，将4种不同剂量（100.0、250.0、500.0和1000.0微克）的该化学物质腹腔注射给成年雄性小鼠组。在十氯酮处理的同时，另一组雄性小鼠接受10.0微克的17β-雌二醇；对照组用芝麻油载体处理。在每日注射化学物质15天后，处死小鼠并对第四脑室的脉络丛进行形态学检查。对照脉络丛的扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查显示立方上皮大量覆盖有微绒毛。十氯酮处理后脉络丛上皮的SEM检查显示出剂量依赖性的细胞改变，这些改变影响了微绒毛和细胞表面。在十氯酮最高剂量处理后，微绒毛不再可见，脉络膜细胞膜显得光滑或有凹痕，并且有腔内碎片增加的迹象。对相同脉络丛细胞的TEM观察显示细胞质空泡化、内质网扩张、线粒体空泡化且嵴断裂以及细胞变性。雌二醇处理后脉络丛的SEM和TEM检查显示出与十氯酮处理后记录的类似细胞改变。讨论了这些数据可能的意义。
