McCall R D, Eroschenko V P
Department of Veterinary Science, University of Idaho, Moscow 83843.
Toxicology. 1988 Feb;48(2):177-89. doi: 10.1016/0300-483x(88)90099-6.
Sexually mature male Japanese quail were treated with either 40.0 micrograms estradiol-17b injected intraperitoneally or 10 ppm, 40 ppm, 80 ppm, 160 ppm, or 200 ppm chlordecone (Kepone) administered in feed for 60 days. Animals were sacrificed on days 10, 30, and 60 and the germinal epithelium was measured morphometrically and examined with transmission electron microscopy. Estradiol had a minimal effect on testicular morphology whereas there was a dose- and time-dependent reduction in germinal epithelium height in all birds following 60 days of chlordecone exposure. Exposure of quail to higher chlordecone doses (80, 160 and 200 ppm) for 30 or 60 days did not alter the ultrastructure of spermatogenic and Sertoli cells in the basal compartments; however, numerous maturing spermatid clumps located in the adluminal compartments of the germinal epithelium exhibited increased vacuolation, cytoplasmic degeneration and desquamation. Since some of the abnormal spermatids were closely associated with Sertoli cells, the possible role of Sertoli cells during pesticide exposure is discussed.
腹腔注射40.0微克的17β-雌二醇，或在饲料中添加10 ppm、40 ppm、80 ppm、160 ppm或200 ppm的十氯酮（开蓬），持续60天。在第10天、30天和60天对动物实施安乐死，并对生精上皮进行形态测量，并采用透射电子显微镜进行检查。雌二醇对睾丸形态的影响极小，而在十氯酮暴露60天后，所有鹌鹑的生精上皮高度均呈现出剂量和时间依赖性降低。鹌鹑暴露于较高剂量（80、160和200 ppm）的十氯酮30天或60天，并未改变基底室中生精细胞和支持细胞的超微结构；然而，位于生精上皮管腔内分泌部的大量成熟精子团显示出空泡化增加、细胞质变性和脱屑现象。由于一些异常精子与支持细胞密切相关，因此讨论了农药暴露期间支持细胞可能发挥的作用。
