Zheng Huanying, Lu Jing, Zhang Yong, Yoshida Hiromu, Guo Xue, Liu Leng, Li Hui, Zeng Hanri, Fang Ling, Mo Yanling, Yi Lina, Chosa Toru, Xu Wenbo, Ke Changwen
Guangdong Provincial Center for Disease Control and Prevention, Panyu District, Guangzhou, China.
Appl Environ Microbiol. 2013 Dec;79(24):7679-83. doi: 10.1128/AEM.02058-13. Epub 2013 Oct 4.
The human-pathogenic viruses in urban sewage have been extensively monitored to obtain information on circulating viruses in human communities. Enteroviruses (EVs) excreted by patients who present with diverse clinical syndromes can remain infectious in the environment for several weeks, and limited data on circulating environmental EVs are available. A 4-year (2009 to 2012) surveillance study was conducted to detect nonpolio enteroviruses (NPEVs) in the urban sewage of Guangzhou city, China. After the viruses in the sewage samples were concentrated and isolated, molecular identification was used to detect and type the NPEVs. During the 4-year study, 17 different NPEV serotypes were identified in the sewage of Guangzhou city. The most common serotypes were echovirus 11 (ECHO11), ECHO6, ECHO7, and ECHO12 and coxsackie group B viruses 5 (CVB5) and CVB3. The predominant serotypes were influenced by spatial and temporal factors and differed each year. CVB5 was commonly detected in 2009 and 2010 but was rarely isolated in 2011 and 2012. In contrast, CVB3 was not observed in 2009 and 2010 but was increasingly detected in 2011 and 2012. Our study provides an overview of the serotype distribution and circulation patterns of NPEVs in the sewage of Guangzhou, China. In the absence of a systematic EV disease surveillance system, the detection and characterization of sewage-borne NPEVs will help us better understand the changes in EV disease trends and the epidemic background of circulating EVs, which could help interpret the EV trends and warn of future outbreaks in this area.
为获取人类社区中传播病毒的信息，已对城市污水中的人类致病病毒进行了广泛监测。出现各种临床综合征的患者排出的肠道病毒（EVs）在环境中可保持传染性数周，而关于环境中传播的肠道病毒的有限数据可用。进行了一项为期4年（2009年至2012年）的监测研究，以检测中国广州市城市污水中的非脊髓灰质炎肠道病毒（NPEVs）。在对污水样本中的病毒进行浓缩和分离后，使用分子鉴定来检测和分型NPEVs。在为期4年的研究中，在广州市污水中鉴定出17种不同的NPEV血清型。最常见的血清型是埃可病毒11型（ECHO11）、ECHO6、ECHO7和ECHO12以及柯萨奇B组病毒5型（CVB5）和CVB3。主要血清型受空间和时间因素影响，每年都有所不同。CVB5在2009年和2010年普遍检测到，但在2011年和2012年很少分离到。相比之下，CVB3在2009年和2010年未观察到，但在2011年和2012年检测到的越来越多。我们的研究概述了中国广州污水中NPEVs的血清型分布和传播模式。在缺乏系统的EV疾病监测系统的情况下，污水中传播的NPEVs 的检测和特征分析将有助于我们更好地了解EV疾病趋势的变化和传播的EVs的流行背景，这有助于解释EV趋势并预警该地区未来的疫情爆发。
