Chen Guangfeng, Shi Xiujuan, Sun Chen, Li Min, Zhou Qing, Zhang Chen, Huang Jun, Qiu Yu, Wen Xiangyi, Zhang Yan, Zhang Yushan, Yang Shuzhang, Lu Lixia, Zhang Jieping, Yuan Qionglan, Lu Jianwei, Xu Guotong, Xue Yunyun, Jin Zibing, Jiang Cizhong, Ying Ming, Liu Xiaoqing
Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
PLoS One. 2013 Oct 3;8(10):e73673. doi: 10.1371/journal.pone.0073673. eCollection 2013.
Human adipose tissue-derived stem cells (ADSCs) are an attractive multipotent stem cell source with therapeutic applicability across diverse fields for the repair and regeneration of acute and chronically damaged tissues. In recent years, there has been increasing interest in ADSC for tissue engineering applications. However, the mechanisms underlying the regulation of ADSC proliferation are not fully understood. Here we show that 47 transcripts are up-regulated while 23 are down-regulated in ADSC compared to terminally differentiated cells based on global mRNA profiling and microRNA profiling. Among the up-regulated genes, the expression of vascular endothelial growth factor (VEGF) is fine-tuned by miR-199a-5p. Further investigation indicates that VEGF accelerates ADSC proliferation whereas the multipotency of ADSC remains stable in terms of adipogenic, chondrogenic and osteogenic potentials after VEGF treatment, suggesting that VEGF may serve as an excellent supplement for accelerating ADSC proliferation during in vitro expansion.
人脂肪组织来源的干细胞（ADSCs）是一种具有吸引力的多能干细胞来源，在急性和慢性受损组织的修复与再生的不同领域具有治疗适用性。近年来，人们对ADSCs在组织工程应用方面的兴趣日益增加。然而，ADSCs增殖调控的潜在机制尚未完全了解。在此我们表明，基于全局mRNA谱和微小RNA谱分析，与终末分化细胞相比，ADSCs中有47个转录本上调，23个转录本下调。在上调基因中，血管内皮生长因子（VEGF）的表达由miR-199a-5p进行微调。进一步研究表明，VEGF可加速ADSCs增殖，而VEGF处理后ADSCs在成脂、成软骨和成骨潜能方面的多能性保持稳定，这表明VEGF可能是体外扩增过程中加速ADSCs增殖的一种极佳补充物。
