Institute of Materials and Biomaterials, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Electromagn Biol Med. 2020 Apr 2;39(2):154-165. doi: 10.1080/15368378.2020.1737809. Epub 2020 Mar 4.
: There are different methods to develop neo-chondral tissues from adipose-derived stem cells (ADSCs). Application of electromagnetic field (EMF) on ADSCs is one of popular approaches, which results in chondrogenesis. If chondrogenic impact of EMF on ADSCs is supposed to be generalized as a protocol in translational medicine field, possible emergence of early or late hypertrophic maturation, mineralization and inflammatory side effects in chondrogenically differentiating ADSCs should be considered.: The advent of chondrogenic and hypertrophic markers by differentiated cells under standard, platelet-rich plasma (PRP)-based or EMF treatments were monitored. Along with monitoring the expressions of chondrogenic markers, inflammatory and hypertrophic markers, VEGF/TNFα secretion, calcium deposition and ALP activity were evaluated.: Accordingly, treatment with %5 PRP results in higher GAG production, enhanced transcription, lowered TNFα and VEGF secretions compared to other treatments. Although PRP up-regulates and in early and late stages of chondrogenesis, respectively, application of EMF + PRP down regulates and while up-regulates and expression.: Comparing our results with previous reports suggests that presented EMF-ELF in this study with = 50 Hz, EMF intensity of less than 30 mT, and 5% PRP (v/v), would facilitate chondrogenesis via mesenchymal stem cells with minor inflammation and hypertrophic maturation. MSCs: mesenchymal stem cells; TGFβ: transforming growth factor-beta; PRP: platelet-rich plasma; ELF-EMF: extremely low-frequency electromagnetic fields; GAGs: glycosaminoglycans; ADSCs: adipose-derived stem cells; VEGF: vascular endothelial growth factor; TNFα: tumor necrosis factor alpha; ALP: alkaline phosphatase.
有不同的方法可以从脂肪来源的干细胞 (ADSCs) 中开发新的软骨组织。电磁场 (EMF) 应用于 ADSCs 是一种热门方法，可导致软骨生成。如果将 EMF 对 ADSCs 的软骨生成影响作为转化医学领域的一种方案来推广，那么在软骨分化的 ADSCs 中可能会出现早期或晚期肥大成熟、矿化和炎症副作用。
监测了标准、富含血小板的血浆 (PRP) 或 EMF 处理下分化细胞中软骨形成和肥大标志物的出现。在监测软骨形成标志物表达的同时，还评估了炎症和肥大标志物、VEGF/TNFα 分泌、钙沉积和 ALP 活性。
因此，与其他处理相比，处理 5%的 PRP 可导致更高的 GAG 产生、增强转录、降低 TNFα 和 VEGF 的分泌。虽然 PRP 在软骨形成的早期和晚期分别上调 和 ，但 EMF + PRP 的应用下调 和 ，同时上调 和 的表达。
将我们的结果与以前的报告进行比较表明，本研究中使用的 50 Hz、小于 30 mT 的 EMF 强度和 5%的 PRP（v/v）的 EMF-ELF 会通过间充质干细胞促进软骨形成，炎症和肥大成熟程度较小。MSCs：间充质干细胞；TGFβ：转化生长因子-β；PRP：富含血小板的血浆；ELF-EMF：极低频电磁场；GAGs：糖胺聚糖；ADSCs：脂肪来源的干细胞；VEGF：血管内皮生长因子；TNFα：肿瘤坏死因子-α；ALP：碱性磷酸酶。
