• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Nonfluorescent negative stain for alanopine dehydrogenase activity on starch gels.

作者信息

Manchenko G P

出版信息

Anal Biochem. 1985 Mar;145(2):308-10. doi: 10.1016/0003-2697(85)90366-5.

DOI:10.1016/0003-2697(85)90366-5
PMID:2409834
Abstract

A two-step procedure for negative staining NADH-dependent alanopine dehydrogenase (ALPDH) activity on starch gels is described, using nitroblue tetrasolium and phenasine methosulfate. This method gives a blue background with unreacted NADH, and white areas where ALPDH is located. Using this technique the first reliable evidence of the existence of true ALPDH isoenzymes is obtained.

摘要

相似文献

1
Nonfluorescent negative stain for alanopine dehydrogenase activity on starch gels.
Anal Biochem. 1985 Mar;145(2):308-10. doi: 10.1016/0003-2697(85)90366-5.
2
An explanation of the achromatic bands produced by peroxidase isozymes in polyacrylamide electrophoresis gels stained for malate dehydrogenase.对在经苹果酸脱氢酶染色的聚丙烯酰胺电泳凝胶中过氧化物酶同工酶产生的无色带的解释。
Electrophoresis. 1992 Jan-Feb;13(1-2):82-6. doi: 10.1002/elps.1150130116.
3
Purification and properties of alanopine dehydrogenase from the adductor muscle of the oyster, Crassostrea gigas (Mollusca, Bivalvia).
Eur J Biochem. 1981 Mar;114(3):615-21. doi: 10.1111/j.1432-1033.1981.tb05188.x.
4
Artifactual staining of proteins on polyacrylamide gels by nitrobluetetrazolium chloride and phenazine methosulfate.
Anal Biochem. 1980 Jan 1;101(1):215-20. doi: 10.1016/0003-2697(80)90063-9.
5
Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea.
Comp Biochem Physiol B Biochem Mol Biol. 2007 Jun;147(2):293-307. doi: 10.1016/j.cbpb.2007.01.018. Epub 2007 Feb 1.
6
Staining normal and experimental motor nerve terminals with tetrazolium salts.
Stain Technol. 1983 Jan;58(1):21-7. doi: 10.3109/10520298309066745.
7
The role of exogenous electron carriers in NAD(P)-dependent dehydrogenase cytochemistry studied in vitro and with a model system of polyacrylamide films.在体外以及利用聚丙烯酰胺膜模型系统研究外源电子载体在NAD(P)依赖脱氢酶细胞化学中的作用。
J Histochem Cytochem. 1982 Jan;30(1):12-20. doi: 10.1177/30.1.6172468.
8
[Effect of nonionic detergents on the results of studies of dehydrogenase and cholinesterase activities under conditions of enzyme electrophoresis].[非离子洗涤剂对酶电泳条件下脱氢酶和胆碱酯酶活性研究结果的影响]
Vopr Med Khim. 1983 Mar-Apr;29(2):48-51.
9
Demonstration of monophenoloxidase activity of tyrosinase after electrophoresis.电泳后酪氨酸酶单酚氧化酶活性的显示。
Biotech Histochem. 1991;66(3):125-30. doi: 10.3109/10520299109110565.
10
Binding region of alanopine dehydrogenase predicted by unbiased molecular dynamics simulations of ligand diffusion.通过对配体扩散的无偏分子动力学模拟预测丙氨酸脱氢酶的结合区域。
J Chem Inf Model. 2013 Oct 28;53(10):2493-8. doi: 10.1021/ci400370y. Epub 2013 Sep 25.