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Automated determination of DNA using the fluorochrome Hoechst 33258.

作者信息

Sterzel W, Bedford P, Eisenbrand G

出版信息

Anal Biochem. 1985 Jun;147(2):462-7. doi: 10.1016/0003-2697(85)90299-4.

DOI:10.1016/0003-2697(85)90299-4
PMID:2409841
Abstract

An automated method for the determination of DNA content in fractions from the alkaline filter elution assay of DNA damage has been developed. DNA-containing fractions are mixed with a fluorochrome (Hoechst 33258) and the DNA concentration is measured fluorometrically in a continuous-flow system. The lower limit of detection is 0.05 micrograms DNA/ml, and the linearity range under the conditions used is 0-8 micrograms DNA/ml. The standard deviation (n = 10) was found to be +/- 0.83%. The results are compared with the manual method.

摘要

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