Cowell J K, Franks L M
J Histochem Cytochem. 1980 Mar;28(3):206-10. doi: 10.1177/28.3.6153398.
A method is described for the rapid, accurate measurement of single cells in situ using microfluorimetry. This method involves a shutter system, which allows irradiation of single cells for fractions of a second and a peak fluorescence intensity recording device. In this way errors due to fluorochrome fading are almost eliminated and standard deviations of less than 5% are obtained. Hoechst 33258 has been used as a quantitative fluorochrome. Optimum fixation and staining conditions on glass and plastic tissue culture vessels are described.
描述了一种使用显微荧光测定法快速、准确地原位测量单细胞的方法。该方法涉及一个快门系统，它允许对单细胞进行几分之一秒的照射以及一个峰值荧光强度记录装置。通过这种方式，几乎消除了由于荧光染料褪色引起的误差，并获得了小于5%的标准偏差。已将Hoechst 33258用作定量荧光染料。描述了在玻璃和塑料组织培养容器上的最佳固定和染色条件。
