Chan-Chable Rahuel J, Martínez-Arce Arely, Mis-Avila Pedro C, Ortega-Morales Aldo I
Departamento de Sistemática y Ecología Acuática Unidad Chetumal, El Colegio de la Frontera Sur Chetumal Quintana Roo México.
Departamento de Enfermedades Transmitidas por Vector y Zoonosis Servicios Estatales de Salud de Quintana Roo Chetumal Quintana Roo México.
Ecol Evol. 2019 Mar 23;9(8):4692-4705. doi: 10.1002/ece3.5073. eCollection 2019 Apr.
Culicidae mosquitoes are potential vectors of pathogens that affect human health. The correct species identification, as well as the discovery and description of cryptic species, is important in public health for the control and management of specific vectors. In the present study, the diversity of anthropophagous mosquitoes in Quintana Roo, at the border between Mexico and Belize, was evaluated using morphological and molecular data (COI-DNA Barcoding). A total of 1,413 adult female specimens were collected, belonging to eight genera and 31 morphospecies. Most species formed well-supported clades. Intraspecific Kimura 2 parameters (K2P) distance average was 0.75%, and a maximum distance of 4.40% was observed for s.l. ABGD method identified 28 entities, while 32 entities were identified with the BIN system. In and a low interspecific genetic distance of 0.1% was observed. One undescribed species belonging to the genus (n. sp.) was discovered, but no clear genetic divergence was found between this species and the closely related species . An intraspecific K2P distance greater than 2.7% was observed in (3.9%), s.l. (4.4%), (3.7%), (3.9%), (5.0%), (4.5%), and in (8.10%); therefore, evidences of cryptic diversity are shown in these species. This study showed that DNA barcodes offer a reliable framework for mosquito species identification in Quintana Roo, except for some closely related species for which it is recommended to use additional nuclear genetic markers such as ITS2, in order to resolve these small discrepancies.
蚊科蚊子是影响人类健康的病原体的潜在传播媒介。正确的物种鉴定以及隐秘物种的发现和描述，对于公共卫生中特定传播媒介的控制和管理至关重要。在本研究中，利用形态学和分子数据（COI-DNA条形码）评估了位于墨西哥和伯利兹边境的金塔纳罗奥州嗜人蚊子的多样性。共采集了1413只成年雌性标本，分属于8个属和31个形态种。大多数物种形成了支持度良好的进化枝。种内Kimura 2参数（K2P）距离平均值为0.75%，并观察到广义上的最大距离为4.40%。ABGD方法鉴定出28个实体，而BIN系统鉴定出32个实体。在[具体物种1]和[具体物种2]中观察到种间遗传距离低至0.1%。发现了一个属于[属名]属的未描述物种（新种），但该物种与近缘物种[近缘物种名]之间未发现明显的遗传分化。在[具体物种3]（3.9%）、广义上的[具体物种4]（4.4%）、[具体物种5]（3.7%）、[具体物种6]（3.9%）、[具体物种7]（5.0%）、[具体物种8]（4.5%）以及[具体物种9]（8.10%）中观察到种内K2P距离大于2.7%；因此，这些物种显示出隐秘多样性的证据。本研究表明，DNA条形码为金塔纳罗奥州蚊子物种鉴定提供了一个可靠的框架，但对于一些近缘物种，建议使用额外的核遗传标记如ITS2，以解决这些微小差异。
