荷花（Burm. f）花的抗菌活性。
Antibacterial activity of Nymphaea nouchali (Burm. f) flower.
机构信息
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, Bangladesh.
出版信息
Ann Clin Microbiol Antimicrob. 2013 Oct 7;12:27. doi: 10.1186/1476-0711-12-27.
BACKGROUND
The present work aimed to find out the antibacterial activity of Nymphaea nouchali flower on human and plant pathogenic bacteria.
METHODS
Antibacterial potency of methanol, acetone, ethyl acetate and petroleum spirit extracts of Nymphaea nouchali flower has been tested against four human pathogenic bacteria Bacillus subtilis (FO 3026) Escherichia coli (IFO 3007), Klebsiella pneumonia (ATTC 10031) and Sarcina lutea (IFO 3232) and one plant pathogenic bacterium Xanthomonas campestris (IAM 1671) by disc diffusion assay. Zone of inhibition produced by different extracts against the test bacteria was measured and compared with standard antibiotic disc.
RESULTS
Methanol extract possessed better antibacterial activity against two pathogenic bacteria, B. subtilis (FO 3026) and S. lutea (IFO 3232) than commercial antibiotic nalidixic acid. Acetone extract showed moderate sensitivity whereas B. subtilis (FO 3026), S. lutea (IFO 3232) and X. campestris (IAM 1671) showed resistance to ethyl acetate and petroleum spirit extracts. The minimum inhibitory concentrations of various extracts were ranged between 128-2048 μgml-1.
CONCLUSIONS
Nymphaea nouchali flower could be a potential candidate for future development of novel broad spectrum antibacterial herbal formulation.
背景
本研究旨在探究荇菜花对人类和植物病原菌的抗菌活性。
方法
采用纸片扩散法，检测甲醇、丙酮、乙酸乙酯和石油醚提取物对枯草芽孢杆菌（FO3026）、大肠杆菌（IFO3007）、肺炎克雷伯菌（ATTC10031）、藤黄微球菌（IFO3232）4 种人源病原菌和 1 种植物病原菌野油菜黄单胞菌（IAM1671）的抑菌活性。并与标准抗生素纸片比较，测量不同提取物对试验菌的抑菌圈直径。
结果
甲醇提取物对枯草芽孢杆菌（FO3026）和藤黄微球菌（IFO3232）这 2 种病原菌的抑菌活性优于市售抗生素萘啶酸。丙酮提取物显示出中等敏感性，而乙酸乙酯和石油醚提取物对枯草芽孢杆菌（FO3026）、藤黄微球菌（IFO3232）和野油菜黄单胞菌（IAM1671）表现出抗性。不同提取物的最低抑菌浓度在 128-2048 μg/ml 之间。
结论
荇菜花可能是未来开发新型广谱抗菌草药制剂的潜在候选药物。
Zotero 插件