P,G, & Research Department of Zoology, Voorhees College, Vellore, Tamilnadu 632001, India.
BMC Complement Altern Med. 2014 Sep 26;14:361. doi: 10.1186/1472-6882-14-361.
In the Indian ayurvedic system of medicine, Nymphaea nouchali is used for the treatment of diabetes, cutaneous diseases, inflammation, liver disorders, urinary disorders, menorrhagia, blenorrhagia, menstruation problem, as an aphrodisiac, and as a bitter tonic. However, despite its traditional usage as an antimicrobial agent, there is no information regarding its effectiveness in infections caused by pathogenic microbes. Hence, we evaluated 70% ethanol extract of the seeds of N. nouchali for its antimicrobial activity.
The antimicrobial activity of the extract at five different concentrations was tested against few common human pathogenic microorganisms by agar disc diffusion assay. The Minimum Inhibitory Concentration of the extract was determined by the modified resazurin method. Streptomycin (10 μg/ml) and amphotericin B (10 μg/ml) were used as standards for antibacterial and antifungal study respectively. Few phenolic compounds were identified and quantified by standard HPTLC technique.
The zone of inhibition was extremely great for P. aeruginosa (25 mm), S. aureus (20 mm) and C. albicans (19 mm). MIC value was the least at 0.03 mg/ml for bacteria: K. pneumoniae, S. dysenteriae and E. coli and 0.31 mg/ml for fungi: C. albicans and T. mentagrophytes. Moreover, through HPTLC analysis few phenolic compounds were quantified, among which catechin content was found to be the highest (3.06%), followed by gallic acid (0.27%) and quercetin (0.04%).
The results therefore clearly indicates that the crude extract from N. nouchali seeds could be used as a potential source of natural antimicrobial agent owing to the presence of the phytoconstituent catechin in abundance along with other active compounds and supports the traditional use of the plant in the treatment of infections.
在印度阿育吠陀医学体系中，芡实属植物 Nymphaea nouchali 被用于治疗糖尿病、皮肤病、炎症、肝脏疾病、尿路疾病、月经过多、淋病、月经问题、作为壮阳药和苦味滋补剂。然而，尽管它作为一种抗菌剂的传统用途，但没有关于其在致病微生物引起的感染中的有效性的信息。因此，我们评估了 N. nouchali 种子的 70%乙醇提取物的抗菌活性。
通过琼脂圆盘扩散法，在五个不同浓度下测试提取物对几种常见的人类致病微生物的抗菌活性。通过改良的 Resazurin 法测定提取物的最小抑菌浓度。链霉素（10μg/ml）和两性霉素 B（10μg/ml）分别用作抗细菌和抗真菌研究的标准。通过标准 HPTLC 技术鉴定和定量几种酚类化合物。
对铜绿假单胞菌（25mm）、金黄色葡萄球菌（20mm）和白色念珠菌（19mm）的抑制圈非常大。MIC 值最低，细菌为 0.03mg/ml，包括肺炎克雷伯菌、痢疾志贺菌和大肠杆菌，真菌为 0.31mg/ml，包括白色念珠菌和须癣毛癣菌。此外，通过 HPTLC 分析定量了几种酚类化合物，其中表儿茶素含量最高（3.06%），其次是没食子酸（0.27%）和槲皮素（0.04%）。
因此，结果清楚地表明，芡实属植物种子的粗提物可能因其富含儿茶素等活性化合物而可作为天然抗菌剂的潜在来源，支持该植物在感染治疗中的传统用途。
