Held W A, Gallagher J F
Biochem Genet. 1985 Apr;23(3-4):281-90. doi: 10.1007/BF00504325.
Rat alpha 2u-globulin and the mouse major urinary proteins (MUP) are encoded by homologous multigene families whose members exhibit diverse tissue-specific, developmental, and hormonal controls of expression. Although their patterns of expression and hormonal control appear to be very similar in many respects, we have found high levels of alpha 2u-globulin mRNA in rat preputial glands, whereas MUP mRNA could not be detected in the male mouse preputial gland. Male and female rat preputial have similar concentrations of alpha 2u-globulin mRNA, suggesting an absence of endocrine regulation as occurs in the liver and lachrymal glands. Two-dimensional polyacrylamide gel electrophoresis of proteins encoded by hybrid-selected alpha 2u-globulin mRNA indicates that the liver and lachrymal translation products have different mobilities. However, many of the preputial gland products comigrate with most or all of the liver and lachrymal products. Among the possibilities suggested by these results is that alpha 2u-globulin genes expressed in liver and lachrymal glands under endocrine control are also expressed constitutively in the preputial gland.
大鼠α2u球蛋白和小鼠主要尿蛋白（MUP）由同源多基因家族编码，其成员表现出不同的组织特异性、发育和激素调控的表达。尽管它们的表达模式和激素调控在许多方面似乎非常相似，但我们发现在大鼠包皮腺中α2u球蛋白mRNA水平很高，而在雄性小鼠包皮腺中未检测到MUP mRNA。雄性和雌性大鼠包皮腺中α2u球蛋白mRNA浓度相似，这表明不存在如肝脏和泪腺中发生的内分泌调节。对杂交选择的α2u球蛋白mRNA编码的蛋白质进行二维聚丙烯酰胺凝胶电泳表明，肝脏和泪腺的翻译产物具有不同的迁移率。然而，许多包皮腺产物与大多数或所有肝脏和泪腺产物迁移到同一位置。这些结果提示的可能性之一是，在内分泌控制下在肝脏和泪腺中表达的α2u球蛋白基因也在包皮腺中组成性表达。
