Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Shaanxi, China.
Anticancer Drugs. 2014 Feb;25(2):174-82. doi: 10.1097/CAD.0000000000000031.
The aim of this paper was to investigate whether sinoporphyrin sodium (DVDMS) could be a novel sonosensitizer in sonodynamic therapy. We used two kinds of leukemia cells (K562, U937) as main tumor cell models and cells (peripheral blood mononuclear cells, spleen lymphocytes) separated from healthy ICR mice as normal cell models. The multivolume spectrophotometer system and fluorescence spectrophotometer were used to determine the spectral characteristics of DVDMS. The uptake of DVDMS by tumor cells and normal cells was measured by flow cytometry. The MTT assay was used to examine the cytotoxicity and sonotoxicity of DVDMS. The absorption spectra showed that DVDMS had five distinct peaks at 359, 514, 548, 580, and 631 nm, respectively, and the maximum peak was at ∼359 nm. The fluorescence emission spectra showed that DVDMS fluorescence emission was at 642 nm. DVDMS showed an advantage of quick cellular uptake and selectively accumulated in tumor cells compared with normal healthy cells. The cytotoxicity of DVDMS by the MTT method was dose dependent, and DVDMS had little cytotoxicity to normal cells. The sonotoxicity of DVDMS showed that in the presence of DVDMS, under appropriate conditions, the cell-damaging effect of ultrasound was significantly enhanced. The present study showed that the newly synthesized sensitizer, DVDMS, under appropriate experiment conditions, can act as a potential sonosensitizer for tumors in sonodynamic therapy.
本文旨在研究血卟啉单甲醚（DVDMS）是否可以作为声动力学治疗中的一种新型声敏剂。我们使用两种白血病细胞（K562、U937）作为主要肿瘤细胞模型，以及从健康 ICR 小鼠分离的细胞（外周血单核细胞、脾淋巴细胞）作为正常细胞模型。采用多容积分光光度计系统和荧光分光光度计测定 DVDMS 的光谱特征。采用流式细胞术测定肿瘤细胞和正常细胞对 DVDMS 的摄取。MTT 法检测 DVDMS 的细胞毒性和声毒性。吸收光谱表明，DVDMS 在 359、514、548、580 和 631nm 处分别有五个明显的峰，最大峰值约为 359nm。荧光发射光谱表明 DVDMS 的荧光发射在 642nm。与正常健康细胞相比，DVDMS 具有快速细胞摄取的优势，并选择性地积聚在肿瘤细胞中。MTT 法测定的 DVDMS 细胞毒性呈剂量依赖性，对正常细胞的细胞毒性较小。DVDMS 的声毒性研究表明，在 DVDMS 的存在下，在适当的条件下，超声的细胞损伤作用明显增强。本研究表明，新合成的敏化剂 DVDMS 在适当的实验条件下，可以作为声动力学治疗中肿瘤的潜在声敏剂。
